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Isolation and characterization of fibrolytic enzymes from sheep rumen bacteria.

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Title Isolation and characterization of fibrolytic enzymes from sheep rumen bacteria.
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Creator Sharma SA, Sharma D, Rana K, Kanwar SS, Mal, G, Singh B.
 
Subject Fibrolytic Bacteria, Plant Biomass, Probiotics, Sheep Rumen
 
Description Not Available
This study reports characterization of fibrolytic
enzymes from rumen bacteria of migratory Gaddi sheep in North West Himalayan Region (NWHR). A total of 70 cellulolytic
bacteria were isolated. Two isolates viz., CDB1 and CDB5 due to
higher secretion of fibrolytic enzymes, namely endoglucanase and
β-glucosidase were studied. The endoglucanase and βglucosidase
activities in isolate CDB1 and isolate CDB5 were found to be
0.720 and 0.696 U/mL and 0.820 and 0.758 U/mL, respectively.
The isolate CDB1showed optimal enzyme production using yeast
extract, while isolate CDB5 used peptone as nitrogen source for
production of the enzyme. The endoglucanase (0.720 and 0.750
U/mL) and βglucosidase (0.710 and 0.722 U/mL) activities in
CDB1 and CDB5 were optimal at 40°C and 50°C, respectively.
Highest level of endoglucanase (0.742 and 0.850 U/mL) and
βglucosidase activities (0.724 and 0.810 U/mL) produced by
CDB1 and CDB5 were detected at pH 6.0 to 7.0, and 6.0 to
8.0, respectively. In view of ability of the isolates to inhibit some
undesirable microorganisms, and activity of enzymes produced
at 40°C and 50°C, it is envisaged that these isolates may have
applications as probiotics and additives in silage making.
Not Available
 
Date 2023-05-24T08:15:56Z
2023-05-24T08:15:56Z
2017-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77722
 
Language English
 
Relation Not Available;
 
Publisher Not Available