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Isolation, identification and molecular characterization of tannase producing Klebsiella sp., from the rumen of migratory goats and sheep

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Title Isolation, identification and molecular characterization of tannase producing Klebsiella sp., from the rumen of migratory goats and sheep
Not Available
 
Creator AR Sahu, B Singh, G Mal, A Padhy, A Kannan, R Bhar
 
Subject Hydrolysable tannin, tannase enzyme, direct fed microbial, 16S rRNA amplification, molecular characterization
 
Description Not Available
Tannase, so called Tannin Acyl Hydrolase (TAH) (E.C.3.1.1.20) is one of the versatile
biocatalyst with several industrial applications. It is solely responsible for the degradation of
hydrolysable tannin thus surpassing the untoward effects of high concentration of tannin
consumption in the gut of small ruminants. In the locality of Palampur the migratory goats and
sheep have the unique property to resist excess amount of tannin consumed by them. This is
because of their ruminal microflora. The concept of ruminal microflora can be used as Direct Fed
Microbial (DFM) gaining momentum now-a-days. Thus in the present study attempts were made
to explore new source of tannase producing microbes from the rumen of goats and sheep. Rumen
samples were collected over several places of Palampur, then processed and finally the most tannin
tolerable cultures were selected. Their genomic DNA were isolated and subjected for 16S rRNA
sequencing to characterize the isolates. From the molecular characterization we identified
Klebsiella sp. as a novel source of tannase from the rumen of these migratory animals. This is the
first report of a Klebsiella sp. from the rumen of migratory small ruminants capable of degrading
forage tannin.
Not Available
 
Date 2023-05-24T08:17:37Z
2023-05-24T08:17:37Z
2015-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77725
 
Language English
 
Relation Not Available;
 
Publisher Not Available