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Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells

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Title Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells
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Creator Not Available
Varadarajan Parthasarathy, Francine Martin, Adrian Higginbottom, Helen Murray, Gregory W. Moseley, Robert C. Read, Gorakh Mal, Rachel Hulme, Peter N. Monk and Lynda J. Partridge
 
Subject CD9; CD63; monocyte fusion; multinucleated giant cell; tetraspanin
 
Description Not Available
Members of the tetraspanin superfamily of proteins are implicated in a variety of complex cell processes including cell fusion. However, the contribution of individual tetraspanins to these processes has proved difficult to define. Here we report the use of recombinant extracellular regions of tetraspanins to investigate the role of specific members of this family in the fusion of monocytes to form multinucleated giant cells (MGC). In contrast to their positive requirement in sperm–egg fusion, previous stud-ies using antibodies and knockout mice have indicated a negative regulatory role for tetraspanins CD9 and CD81 in this process. In an in vitro model of fusion using human monocytes, we have confirmed observations that antibodies to CD9 and CD81 enhance MGC formation; however, in contrast to previous investigations, we found that all members of a panel of antibodies to CD63 inhibited fusion. Moreover, recombinant proteins corresponding to the large extracellular domains (EC2s) of CD63 andCD9 inhibited MGC formation, whereas the EC2s of CD81 and CD151had no effect. The potent inhibition of fusion and binding of labelledCD63 EC2 to monocytes under fusogenic conditions suggest a direct interaction with a membrane component required for fusion. Our findings indicate that the tetraspanins CD9, CD63 and CD81 are all involved in MGC formation, but play distinct roles.
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Date 2023-05-25T08:23:05Z
2023-05-25T08:23:05Z
2008-08-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/77751
 
Language English
 
Relation Not Available;
 
Publisher Blackwell Publishing Ltd