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Role of protein repair enzymes in oxidative stress survival and virulence of Salmonella

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Title Role of protein repair enzymes in oxidative stress survival and virulence of Salmonella
Not Available
 
Creator Arijit Shome
Ratanti Sarkhel
Shekhar Apoorva
Sonu Sukumaran Nair
Tapan Kumar Singh Chauhan
Sanjeev Kumar Bhure
Manish Mahawar
 
Subject Oxidative stress
Protein damage
Protein repair
Salmonella
Poultry
 
Description Not Available
Purpose: Proteins are the principal biomolecules in bacteria that are affected by the oxidants produced by the
phagocytic cells. Most of the protein damage is irreparable though few unfolded proteins and covalently modified
amino acids can be repaired by chaperones and repair enzymes respectively. This study reviews the three protein
repair enzymes, protein L-isoaspartyl O-methyl transferase (PIMT), peptidyl proline cis-trans isomerase (PPIase), and
methionine sulfoxide reductase (MSR).
Methods: Published articles regarding protein repair enzymes were collected from Google Scholar and PubMed.
The information obtained from the research articles was analyzed and categorized into general information about
the enzyme, mechanism of action, and role played by the enzymes in bacteria. Special emphasis was given to the
importance of these enzymes in Salmonella Typhimurium.
Results: Protein repair is the direct and energetically preferred way of replenishing the cellular protein pool without
translational synthesis. Under the oxidative stress mounted by the host during the infection, protein repair becomes
very crucial for the survival of the bacterial pathogens. Only a few covalent modifications of amino acids are
reversible by the protein repair enzymes, and they are highly specific in activity. Deletion mutants of these enzymes
in different bacteria revealed their importance in the virulence and oxidative stress survival.
Conclusion: PIMT repairs isoaspartate residues, PPiase catalyzes the conversion of cis-trans forms of proline residues,
while MSR repairs oxidized methionine (Met) residues in the proteins. These repair enzymes maintain the activities
of the target protein(s), thus aid in bacterial survival and virulence. The interventions which can interfere with this
mechanism could be used for the development of novel therapeutics
Not Available
 
Date 2023-05-26T03:30:31Z
2023-05-26T03:30:31Z
2020-09-17
 
Type Review Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77760
 
Language English
 
Relation Not Available;
 
Publisher BMCres