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Putative virulence traits and pathogenicity of Vibrio cholerae Non-O1, Non-O139 isolates from surface waters in Kolkata, India

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Title Putative virulence traits and pathogenicity of Vibrio cholerae Non-O1, Non-O139 isolates from surface waters in Kolkata, India
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Creator Bag PK, Bhowmik P, Hajra TK, Ramamurthy T, Sarkar P, Majumder M, Chowdhury G, Das SC
 
Subject Vibrio cholerae NonO1 NonO139 Virulence traits surface water
 
Description Acted as working team member
Vibrio cholerae non-O1, non-O139 was isolated from natural surface waters from different sites sampled
in diarrhea endemic zones in Kolkata, India. Twenty-one of these isolates were randomly selected and
included in the characterization. The multiserogroup isolates were compared by their virulence traits with
a group of clinical non-O1, non-O139 isolates from the same geographic area. Of the 21 environmental
isolates, 6 and 14 strains belonged to Heiberg groups I and II, respectively. Three of the environmental
isolates showed resistance to 2,2-diamine-6,7-diisopropylpteridine phosphate. All of the non-O1, nonO139 strains were positive for toxR, and except for one environmental isolate, none of them were positive
for tcpA in the PCR assay. None of the isolates were positive for genes encoding cholera toxin (ctxA),
heat-stable toxin (est), heat-labile toxin (elt), and Shiga toxin variants (stx) of Escherichia coli. Additionally, except for one environmental isolate (PC32), all were positive for the gene encoding El Tor hemolysin
(hly). The culture supernatants of 86% (18 of 21) of the environmental isolates showed a distinct cytotoxic
effect on HeLa cells, and some of these strains also produced cell-rounding factor. The lipase, protease,
and cell-associated hemagglutination activities and serum resistance properties of the environmental and
clinical isolates did not differ much. However, seven environmental isolates exhibited very high hemolytic
activities (80 to 100%), while none of the clinical strains belonged to this group. The environmental
isolates manifested three adherence patterns, namely, carpet-like, diffuse, and aggregative adherence, and
the clinical isolates showed diffuse adherence on HeLa cells. Of the 11 environmental isolates tested for
enteropathogenic potential, 8 (73%) induced positive fluid accumulation (>100) in a mouse model, and the
reactivities of these isolates were comparable to those of clinical strains of non-O1, non-O139 and
toxigenic O139 V. cholerae. Comparison of the counts of the colonized environmental and clinical strains
in the mouse intestine showed that the organisms of both groups had similar colonizing efficiencies. These
findings indicate the presence of potentially pathogenic V. cholerae non-O1, non-O139 strains in surface
waters of the studied sites in Kolkata.
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Date 2023-05-26T03:36:12Z
2023-05-26T03:36:12Z
2008-01-01
 
Type Article
 
Identifier Bag PK, Bhowmik P, Hajra TK, Ramamurthy T, Sarkar P, Majumder M, Chowdhury G, Das SC. Putative virulence traits and pathogenicity of Vibrio cholerae Non-O1, Non-O139 isolates from surface waters in Kolkata, India. Appl Environ Microbiol. 2008 Sep;74(18):5635-44. doi: 10.1128/AEM.00029-08. Epub 2008 Jul 18. PMID: 18641168; PMCID: PMC2547030.
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http://krishi.icar.gov.in/jspui/handle/123456789/77765
 
Language English
 
Relation Not Available;
 
Publisher PMC American Society for Microbiology (ASM)