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Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India

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Title Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India
Not Available
 
Creator Yadav JP, Das SC, Dhaka Pankaj, Mukhopadhyay AK, Chowdhury G, Naskar S and Malik SVS
 
Subject Not available
 
Description Worked as a team member
Clostridium perfringens is one of the most important globally recognised gastroenteric pathogen in
humans as well as animals. The present study was aimed to know the similarities/divergence among
C. perfringens type A isolates of human and animal origin using the pulsed-field gel electrophoresis
(PFGE) as a molecular tool. The enterotoxic isolates obtained by screening of human diarrhoeal cases
(n = 130), diarrhoeal cases of pig (n = 52) and goat (n = 50), meat samples viz., pork (n = 59) and chevon
(n = 57) were characterized by standard cultural and biochemical methods followed by PCR Assays.
Accordingly, a total of 11 C. perfringens type A characterized isolates (16S rRNA+
, cpa+
, cpb2
+ and cpe+
)
recovered from human diarrhoeal cases (n = 3); diarrhoeal cases of pig (n = 2) and goat (n = 2); meat samples viz. pork (n = 2) and chevon (n = 2) were examined employing PFGE. The observed clustering pattern
in PFGE analysis showed the relatedness between isolates from diarrhoeal goat and chevon (90–100%);
diarrhoeal pig and pork (65–68%); moreover, isolates from human diarrhoeal cases were exhibiting lineage to cases from goat and pig diarrhoea as well pork and chevon by 62–68% relatedness. The outcome
of the present study indicates the probable contamination of this pathogen to the human food chain
through faeces from suspected food animals viz. goat and pig and their improperly cooked meat.
2017 Faculty of Veterinary Medicine, Cairo University. Production and hosting by Elsevier B.V. This is an
open access article under the CC BY-NC-ND license
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Date 2023-05-29T04:49:12Z
2023-05-29T04:49:12Z
2018-06-01
 
Type Article
 
Identifier Yadav JP, Das SC, Dhaka P, Mukhopadhyay AK, Chowdhury G, Naskar S, Malik SS. Pulsed-field gel electrophoresis of enterotoxic Clostridium perfringens type A isolates recovered from humans and animals in Kolkata, India. Int J Vet Sci Med. 2017 Dec 15;6(1):123-126. doi: 10.1016/j.ijvsm.2017.11.002. PMID: 30255089; PMCID: PMC6147381.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77819
 
Language English
 
Relation Not Available;
 
Publisher Taylor and Francis Ltd.