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Molecular Characterization and Antibiotic Resistance of Pathogenic Vibrio parahaemolyticus isolated from Oyster in Coastal Bengal, India and their potential for implication in food chain

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Title Molecular Characterization and Antibiotic Resistance of Pathogenic Vibrio parahaemolyticus isolated from Oyster in Coastal Bengal, India and their potential for implication in food chain
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Creator Parthasarathy S, Das SC, Kumar A, Chowdhury G, Dutta S and Mukhopadhyay AK
 
Subject Antibiotic resistance; Oyster; PFGE; Pandemic; Vibrio parahaemolyticus
 
Description Worked as a team member
Vibrio parahaemolyticus is one of the leading causes of diarrhoea and gastroenteritis in human on consumption of raw or insufficiently cooked seafood. This study was aimed at isolating and characterizing the pathogenic and pandemic V. parahaemolyticus from oysters (n = 90) in coastal parts of West Bengal, India; their antibiotic resistance and potential for involvement in the food chain. During bacteriological culture, typical V. parahaemolyticus colony was recovered in 88.9% samples followed by presumptive identification in 71 (78.9%) samples by characteristic biochemical (K/A) test. All the presumptive isolates (n = 71) were confirmed by species specific Vp-toxR PCR assay. Of these, 10 (14.08%) were tdh+ and none for the trh. Further, 5 (50%) of these tdh+ isolates were found to carry the pandemic potential gene in PGS-PCR assay; however, none in GS-PCR. Majority (80%) of these pathogenic (tdh+) isolates belonged to pandemic serovars (OUT: KUT; OUT: K24; O1: KUT; O1:K25; O10: KUT) and only 20% to non-pandemic serovars (OUT: K15; O9:K17). All the isolates (100%) exhibited resistance to cefpodoxime followed by ampicillin and cefotaxime (90%), ceftizoxime (60%), tetracycline (50%), ceftriaxone (40%), ciprofloxacin and nalidixic acid (10% each). Overall, the study findings suggested that 11.1% (10/90) of commonly marketed oysters in this area were harbouring pathogenic V. parahaemolyticus. Moreover, 5.5% (5/90) of the oyster population were harbouring pandemic strains of this pathogen. Besides, the pathogenic isolates from oysters were exhibiting a considerable genetic relatedness (53 to 70%) to human clinical isolates in PFGE analysis that relates to a substantial public health risk. Further, their multidrug resistance added gravity to the antimicrobial resistance (AMR), a globally growing public health threat and this is a critical area of concern especially during the treatment of foodborne gastroenteritis.
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Date 2023-05-29T04:50:54Z
2023-05-29T04:50:54Z
2021-01-01
 
Type Article
 
Identifier Parthasarathy S, Das SC, Kumar A, Chowdhury G, Miyoshi SI, Dutta S, Mukhopadhyay AK. Molecular characterization and antibiotic resistance of Vibrio parahaemolyticus from Indian oyster and their probable implication in food chain. World J Microbiol Biotechnol. 2021 Aug 5;37(8):145. doi: 10.1007/s11274-021-03113-3. PMID: 34351514; PMCID: PMC8339392.
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http://krishi.icar.gov.in/jspui/handle/123456789/77820
 
Language English
 
Relation Not Available;
 
Publisher PMC