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Differentiation of sheep pox and goat poxviruses by sequence analysis and PCR-RFLP of P32 gene

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Title Differentiation of sheep pox and goat poxviruses by sequence analysis and PCR-RFLP of P32 gene
Not Available
 
Creator Hosamani M, Mondal B, Tembhurne PA, Bandyopadhyay SK, Singh RK, Rasool TJ
 
Subject sheep pox virus
goat poxvirus
Sequence analysis
 
Description Not Available
Sheep pox and Goat pox are highly contagious viral diseases of small ruminants. These diseases were earlier thought to be caused by a single species of virus, as they are serologically indistinguishable. P32, one of the major immunogenic genes of Capripoxvirus, was isolated and Sequenced from two Indian isolates of goat poxvirus (GPV) and a vaccine strain of sheep poxvirus (SPV). The sequences were compared with other P32 sequences of capripoxviruses available in the database. Sequence analysis revealed that sheep pox and goat poxviruses share 97.5 and 94.7% homology at nucleotide and amino acid level, respectively. A major difference between them is the presence of an additional aspartic acid at 55th position of P32 of sheep poxvirus that is absent in both goat poxvirus and lumpy skin disease virus. Further, six unique neutral nucleotide substitutions were observed at positions 77, 275, 403, 552, 867 and 964 in the sequence of goat poxvirus, which can be taken as GPV signature residues. Similar unique nucleotide signatures could be identified in SPV and LSDV sequences also. Phylogenetic analysis showed that members of the Capripoxvirus could be delineated into three distinct clusters of GPV, SPV and LSDV based on the P32 genomic sequence. Using this information, a PCR-RFLP method has been developed for unequivocal genomic differentiation of SPV and GPV.
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Date 2023-05-29T10:47:28Z
2023-05-29T10:47:28Z
2004-08-01
 
Type Article
 
Identifier Differentiation of sheep pox and goat poxviruses by sequence analysis and PCR-RFLP of P32 gene
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77846
 
Language English
 
Relation Not Available;
 
Publisher Springer link