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Development and validation of multiplex PCR based molecular serotyping of Salmonella serovars associated with poultry in India

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Title Development and validation of multiplex PCR based molecular serotyping of Salmonella serovars associated with poultry in India
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Creator K Mohanapriya
Himani Agri
Subbaiyan Anbazhagan
Damini Khawaskar
Varsha Jayakumar
Michael V Lalrinzuala
K M Himani
I Sophia
Asok K Mariappan
Abhishek
Viswas Konasagara Nagaleekar
Dharmendra K Sinha
Pallab Chaudhuri
Vinod K Chaturvedi
Bhoj R Singh
Prasad Thomas
 
Subject India
Molecular serotyping
PCR
 
Description Not Available
Salmonella species are Gram-negative bacteria with more than 2600 serovars. Among these serovars, many are associated with various diseases in livestock and humans. White Kauffman Le-Minor (WKL) serotyping scheme applies specific serum to determine the serovars of Salmonella. Recent studies have applied molecular methods for serovar predictions. These methods include PCR, hybridization and sequence data to detect/predict serovar-specific genetic elements. Among these, PCR is a robust method if the unique genetic element is already known. Within this context, also involving novel primers, two multiplex PCR assays were standardized to detect six important Salmonella serovars viz. Typhimurium, Enteritidis, Kentucky, Infantis, Virchow and Gallinarum associated with poultry in India. The developed PCR assays showed targeted serovar specificity. Serial dilution experiments of both kit-based and crude lysate DNA preparations indicated similar applicability of both methods for testing from pure cultures. Further the developed assays were validated with 25 recent field isolates to confirm the applicability in routine diagnosis. The PCR assay could predict all the targeted serovars (17/25) with 100% specificity (CI-95%; 0.63-1). Molecular serotyping can reduce the number of serum used incomparison to the conventional serotyping which involves more random application of serum.
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Date 2023-05-31T09:31:15Z
2023-05-31T09:31:15Z
2023-03-30
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77936
 
Language English
 
Relation Not Available;
 
Publisher Not Available