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An optimized protocol for purification of virus-like particles of foot-and-mouth disease virus produced in the baculovirus expression system

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Title An optimized protocol for purification of virus-like particles of foot-and-mouth disease virus produced in the baculovirus expression system
Not Available
 
Creator A Madhavan
V V Dhanesh
D P R Selvaraj
S Gopinath
B P Sreenivasa
R P Tamil Selvan
A Sanyal
S Thiyagarajan
P Saravanan
 
Subject foot-and-mouth disease
VLPs
Purification
 
Description Not Available
Foot-and-mouth disease is a highly endemic, economically significant disease of livestock population
across the world. Higher antigenic variation and short-lived immunity possess the threat of its recurrent
incidence. Developments are underway to produce bio-safe and more stable vaccines using virus-like
particles to enhance immunity duration without handling live infectious viruses. Besides this, VLPs
possess an inherent property of differentiation of infected and vaccinated animals (DIVA) as they do not
carry any non-structural proteins along with them. Here we demonstrate such a bio-safe platform to
produce VLPs using a baculovirus expression system and its efficient purification. The purification
protocol combines the process of filtration and isodensity centrifugation to separate the assembled VLPs
from the host cellular components and demonstrated the presence of pure and concentrated VLPs of
expected buoyant density for further structural and functional studies.
Not Available
 
Date 2023-05-30T04:27:27Z
2023-05-30T04:27:27Z
2021-09-09
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77878
 
Language English
 
Relation Not Available;
 
Publisher The pharma innovation