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Molecular characterization and antimicrobial resistance profile of Clostridium perfringens type A isolates from humans, animals, fish and their environment

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Title Molecular characterization and antimicrobial resistance profile of Clostridium perfringens type A isolates from humans, animals, fish and their environment
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Creator 16. Yadav JP, Das SC, Pankaj Dhaka, Deepthi Vijay, Manesh Kumar, Asish Kumar Mukhopadhyay, Goutam Chowdhury, Pranav Chauhan, Rahul Singh, Kuldeep Dhama.
 
Subject Clostridium perfringens A, molecular characterization, human, animal, fish, environment
 
Description Worked as a team member
The study was aimed to characterize, and determine antibiogram of C. perfringens type A isolated from the feces of human and animal diarrhoeal cases, as well as healthy animals, meat of pigs and goats, gills and intestine of fish and samples from fish pond. A total of 460 samples, including human diarrhoeal cases (n = 130); diarrhoeal cases of pig (n = 52) and goat (n = 50); fecal samples from healthy pig (n = 50) and goat (n = 50); meat samples viz. pork meat (n = 52); goat meat (n = 50) and fish including their environmental sources (n = 26) were used for isolation and identification of C. perfringens type A. All the biochemically confirmed isolates were positive for species-specific 16S rRNA and cpa genes by PCR assays. Toxinotyping of C. perfringens type A isolates showed that overall prevalence of C. perfringens type A with only cpa+ gene was 43.2%; with cpa+ and cpb2+ genes was 45.4%; with cpa+ and cpe+ genes was 4.9%; however, with cpa+, cpb2+and cpe+ genes was 6.6%. Antimicrobial susceptibility testing revealed that 83.7% of isolates were resistant to three or more antibiotics.
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Date 2023-05-30T04:33:49Z
2023-05-30T04:33:49Z
2017-01-01
 
Type Article
 
Identifier Yadav, Jay Prakash, et al. "Molecular characterization and antimicrobial resistance profile of Clostridium perfringens type A isolates from humans, animals, fish and their environment." Anaerobe 47 (2017): 120-124.
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http://krishi.icar.gov.in/jspui/handle/123456789/77883
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER