Molecular characterization and antimicrobial resistance profile of Clostridium perfringens type A isolates from humans, animals, fish and their environment
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Title |
Molecular characterization and antimicrobial resistance profile of Clostridium perfringens type A isolates from humans, animals, fish and their environment
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Creator |
16. Yadav JP, Das SC, Pankaj Dhaka, Deepthi Vijay, Manesh Kumar, Asish Kumar Mukhopadhyay, Goutam Chowdhury, Pranav Chauhan, Rahul Singh, Kuldeep Dhama.
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Subject |
Clostridium perfringens A, molecular characterization, human, animal, fish, environment
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Description |
Worked as a team member
The study was aimed to characterize, and determine antibiogram of C. perfringens type A isolated from the feces of human and animal diarrhoeal cases, as well as healthy animals, meat of pigs and goats, gills and intestine of fish and samples from fish pond. A total of 460 samples, including human diarrhoeal cases (n = 130); diarrhoeal cases of pig (n = 52) and goat (n = 50); fecal samples from healthy pig (n = 50) and goat (n = 50); meat samples viz. pork meat (n = 52); goat meat (n = 50) and fish including their environmental sources (n = 26) were used for isolation and identification of C. perfringens type A. All the biochemically confirmed isolates were positive for species-specific 16S rRNA and cpa genes by PCR assays. Toxinotyping of C. perfringens type A isolates showed that overall prevalence of C. perfringens type A with only cpa+ gene was 43.2%; with cpa+ and cpb2+ genes was 45.4%; with cpa+ and cpe+ genes was 4.9%; however, with cpa+, cpb2+and cpe+ genes was 6.6%. Antimicrobial susceptibility testing revealed that 83.7% of isolates were resistant to three or more antibiotics. Not Available |
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Date |
2023-05-30T04:33:49Z
2023-05-30T04:33:49Z 2017-01-01 |
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Type |
Article
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Identifier |
Yadav, Jay Prakash, et al. "Molecular characterization and antimicrobial resistance profile of Clostridium perfringens type A isolates from humans, animals, fish and their environment." Anaerobe 47 (2017): 120-124.
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/77883 |
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Language |
English
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Not Available;
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