Shatavarin-IV saponin adjuvant elicits IgG and IgG2b responses against Staphylococcus aureus bacterin in a murine model
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Title |
Shatavarin-IV saponin adjuvant elicits IgG and IgG2b responses against Staphylococcus aureus bacterin in a murine model
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Creator |
Arivukarasu Palanisamy, Rinku Sharma, Prithvi Pal Singh, Upendra Sharma, Rajendra Damu Patil, Gorakh Mal, Birbal Singh
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Subject |
Shatavarin-IV (S-IV) isolation and characterization, Asparagus adscendens fruit, Inactivated S. aureus (Bacterin), Shatavarin-IV adjuvant, Immune response
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Description |
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Asparagus adscendens Roxb. also known as “safed musli” or “shatavari” is a medicinal plant commonly found in South Asian countries. Shatavari is effective for the treatment of gastric ul cers, renal stones, bronchitis, diabetes, diabetic neuropathy, irritable bowel syndrome, alcohol withdrawal and has reported immunostimulatory effects. In this study, the adjuvant potential of Shatavarin-IV saponin against Staphylococcus aureus bacterin in mice was investigated. Shatavarin-IV was evaluated for its toxicity and immunomodulatory potential against S. aureus bacterin in mice. Cellular and humoral immune responses were assessed. Shatavarin-IV was isolated from the fruit extract of Asparagus adscendens. The confirmation of the isolated molecule as Shatavarin-IV was done via TLC-based comparison with the standard molecule. Further, the structure was confirmed by using extensive spectroscopic analyses and comparing the observed data with literature reports. It was found safe up to the dose of 0.1 mg in the mice model. Shatavarin-IV adjuvant elicited IgG and IgG2b responses at the dose of 40 μg against S. aureus bacterin. However, the cell-mediated immune response was lesser as compared with the com mercial Quil-A saponin . We demonstrated that Shatavarin-IV saponin adjuvant produced an optimum humoral immune response against S. aureus bacterin. These results highlight the po tential of Shatavarin-IV as an adjuvant in a combination adjuvant in vaccine formulations for induction of potent immune response. Not Available |
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Date |
2023-05-30T04:36:09Z
2023-05-30T04:36:09Z 2023-01-01 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/77885 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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