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Microarray analysis of gene expression in parthenotes and in vitro–derived goat embryos

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Title Microarray analysis of gene expression in parthenotes and in vitro–derived goat embryos
Not Available
 
Creator Singh R
Kumar K
Mahapatra PS
Kumar M
Agarwal P
Bhure SK
Malakar D
Bhanja SK
Bag S.
 
Subject Gene expression
Goat embryo
IVF
Microarray analysis
Parthenogenesis
 
Description Not Available
The present work was carried out to investigate the global gene expression profile to search differentially expressed candidate transcripts between parthenogenetic and in vitro-fertilized (IVF) caprine morula. For this study, total RNA was isolated from diploid parthenogenetic and IVF embryos, and complementary DNA was synthesized. Microarray and relative real-time polymerase chain reaction analysis were performed to check global gene expression profile and validation, respectively. According to the microarray analysis, the total number of upregulated (UR) and downregulated (DR) genes was 613 and 220, respectively in diploid parthenogenetic morula as compared with IVF morula. The number of genes showing about two-, two- to five-, five- to 10-, 10- to 20-, and above 20-fold UR and DR genes was 147, 229, 122, 59, and 56 and 94, 73, 18, 13, and 22, respectively. Five UR genes validated (PTEN, PHF3, CTNNB1, SELK, and NPDC1) and all of them were significantly higher in parthenotes, which was in accordance with microarray results, whereas the expression of DR (AURKC and KLF15) genes were downregulated in parthenotes as observed in microarray results but the difference was not significant (P < 0.05). In conclusion, our findings demonstrate differential expression of a large number of genes in parthenotes compared with IVF embryos, which may be the reason for aberrant parthenogenetic embryo development in caprine species.
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Date 2023-05-30T03:59:37Z
2023-05-30T03:59:37Z
2014-01-11
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77856
 
Language English
 
Relation Not Available;
 
Publisher Elsevier