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Reprogramming of fetal cells by avian EE for generation of pluripotent stem cell like cells in caprine.

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Title Reprogramming of fetal cells by avian EE for generation of pluripotent stem cell like cells in caprine.
Not Available
 
Creator Parul Bharadwaj
Kuldeep Kumar
Renu Singh
Gopal Puri
T. Yasotha
Manish Kumar
Sanjeev Bhure
B.C. Das
M. Sarkar
Sadhan Bag.
 
Subject Caprine
Fetal bovine serum
Fetal cells
Reprogramming
Extract Egg
 
Description Not Available
The present work was carried out to study the ability of avian "Extract Egg" (EE) for reprogramming caprine fetal cells. The isolated caprine fetal cells were cultured in stem cell media supplemented with different percentages of either EE or FBS. The results indicated that the supplementation of 2-4% EE formed lesser but larger size stem cell like cell colonies as compared to 6% or 10% EE. The expression of pluripotent genes were comparatively higher in colonies developed in 2% or 4% as compared to 6% or 10% EE. Further, immunocytochemistry revealed that the colonies developed in all percentage of EE expressed pluripotent markers like Oct4, Nanog, TRA-1-60 and TRA-1-81. Our findings indicated that avian EE has the potentiality to reprogram caprine fetal cells into embryonic state which may help in generation of pluripotent stem cells without using viral vector.
Not Available
 
Date 2023-05-30T04:26:55Z
2023-05-30T04:26:55Z
2013-07-04
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77877
 
Language English
 
Relation Not Available;
 
Publisher Elsevier