Effect of actin polymerization inhibitor during oocyte maturation on parthenogenetic embryo development and their ploidy in Capra hircus
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Title |
Effect of actin polymerization inhibitor during oocyte maturation on parthenogenetic embryo development and their ploidy in Capra hircus
Not Available |
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Creator |
Ranjan R.
Singh R. K. Yasotha T. Kumar Manish. Puri Gopal. Kumar Kuldeep Singh Renu Bhure Sanjeev Malakar D. Bhanja S. K. Sarkar M. Das B. C. Bag Sadhan. |
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Subject |
cytochalasin B
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Description |
Not Available
This study was designed to observe the effect of cytochalasin B (CCB) concentrations on ploidy and early development of parthenogenetic embryos in a caprine species. Caprine oocytes were matured in the presence of different concentrations of CCB (5, 10, 15, and 20 μg/ml) and activated by 7% ethanol followed by incubation with 2 mM DMAP. For embryos fertilized in vitro, oocytes were matured in maturation medium without CCB. The cleavage rate and further embryo development were significantly higher (P < 0.05) when oocytes were treated in this way. The percentage of embryos showed higher diploid values in 15 μg/ml CCB (83.66 ± 1.13), followed by 20 (72.22 ± 1.22), 10 (68.57 ± 1.17), and 5 μg/ml (62.00 ± 2.48). These results indicate that CCB with a concentration of 15 μg/ml in maturation medium can be used for the production of diploid parthenogenetic embryos in the caprine species. Not Available |
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Date |
2023-05-31T05:41:55Z
2023-05-31T05:41:55Z 2013-07-12 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/77923 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer
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