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Effect of actin polymerization inhibitor during oocyte maturation on parthenogenetic embryo development and their ploidy in Capra hircus

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Title Effect of actin polymerization inhibitor during oocyte maturation on parthenogenetic embryo development and their ploidy in Capra hircus
Not Available
 
Creator Ranjan R.
Singh R. K.
Yasotha T.
Kumar Manish.
Puri Gopal.
Kumar Kuldeep
Singh Renu
Bhure Sanjeev
Malakar D.
Bhanja S. K.
Sarkar M.
Das B. C.
Bag Sadhan.
 
Subject cytochalasin B
 
Description Not Available
This study was designed to observe the effect of cytochalasin B (CCB) concentrations on ploidy and early development of parthenogenetic embryos in a caprine species. Caprine oocytes were matured in the presence of different concentrations of CCB (5, 10, 15, and 20 μg/ml) and activated by 7% ethanol followed by incubation with 2 mM DMAP. For embryos fertilized in vitro, oocytes were matured in maturation medium without CCB. The cleavage rate and further embryo development were significantly higher (P < 0.05) when oocytes were treated in this way. The percentage of embryos showed higher diploid values in 15 μg/ml CCB (83.66 ± 1.13), followed by 20 (72.22 ± 1.22), 10 (68.57 ± 1.17), and 5 μg/ml (62.00 ± 2.48). These results indicate that CCB with a concentration of 15 μg/ml in maturation medium can be used for the production of diploid parthenogenetic embryos in the caprine species.
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Date 2023-05-31T05:41:55Z
2023-05-31T05:41:55Z
2013-07-12
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77923
 
Language English
 
Relation Not Available;
 
Publisher Springer