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Development of N-gene based PCR-ELISA for the detection of Peste des petits ruminants virus (PPRV) in clinical samples.

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Title Development of N-gene based PCR-ELISA for the detection of Peste des petits ruminants virus (PPRV) in clinical samples.
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Creator P Saravanan, R P Singh, V Balamurugan, P Dhar, B P Sreenivasa, D Muthuchelvan, A Sen, A G Aleyas, R K Singh, S K Bandyopadhyay
 
Subject clinical samples; diagnosis, differential diagnosis; PCR-ELISA; peste-des-petits-ruminants; Peste-des-petits-ruminants virus; rinderpest; Rinderpest virus; RT-PCR; sandwich ELISA
 
Description Not Available
A highly sensitive N gene-based PCR-ELISA for the detection of Peste-des-petits-ruminants virus (PPRV) was developed. The RT-PCR yielded a digoxigenin (DIG)-labeled product of 336 bp comprising a sequence from PPRV N gene, which was then detected by ELISA. The assay could detect the viral RNA in PPRV-infected tissue culture fluids with a titer as low as 0.1 TCID50/ml. The assay is 10,000 times more sensitive than a classical RT-PCR combined with agarose gel electrophoresis. The assay could detect the virus in the clinical samples, which were negative by conventional sandwich ELISA (S-ELISA). The percentage positivity of the assay in detecting the virus in clinical samples was 66.2% compared to 48.6% for S-ELISA. The assay was more sensitive than S-ELISA also in detecting the virus in early as well as late phases of the disease. In addition, the assay could also be used for differential diagnosis of PPRV and Rinderpest virus (RPV).
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Date 2023-05-31T05:43:26Z
2023-05-31T05:43:26Z
2004
 
Type Research Paper
 
Identifier Saravanan P, Singh RP, Balamurugan V, Dhar P, Sreenivasa BP, Muthuchelvan D, Sen A, Aleyas AG, Singh RK, Bandyopadhyay SK. Development of a N gene-based PCR-ELISA for detection of Peste-des-petits-ruminants virus in clinical samples. Acta Virol. 2004;48(4):249-55. PMID: 15745048.
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http://krishi.icar.gov.in/jspui/handle/123456789/77924
 
Language English
 
Relation Not Available;
 
Publisher AE PRESS