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Global gene expression profile of peripheral blood mononuclear cells challenged with Theileria annulata in crossbred and indigenous cattle

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Title Global gene expression profile of peripheral blood mononuclear cells challenged with Theileria annulata in crossbred and indigenous cattle
Not Available
 
Creator Kumar A
Gaur GK
Gandham RK
Panigrahi M
Ghosh S
Saravanan BC
Bhushan B
Tiwari A
Sulabh S
Priya B
Asaf VNM
Gupta JP
Wani SA
Sahu AR
Sahoo AP
 
Subject Theileria annulata Crossbred Tharparkar cattle Microarray
 
Description Not Available
Bovine tropical theileriosis is an important haemoprotozoan disease associated with high rates of morbidity and
mortality particularly in exotic and crossbred cattle. It is one of the major constraints of the livestock development programmes in India and Southeast Asia. Indigenous cattle (Bos indicus) are reported to be comparatively
less affected than exotic and crossbred cattle. However, genetic basis of resistance to tropical theileriosis in indigenous cattle is not well documented. Recent studies incited an idea that differentially expressed genes in exotic
and indigenous cattle play significant role in breed specific resistance to tropical theileriosis. The present study
was designed to determine the global gene expression profile in peripheral blood mononuclear cells derived
from indigenous (Tharparkar) and cross-bred cattle following in vitro infection of T. annulata (Parbhani strain).
Two separate microarray experiments were carried out each for cross-bred and Tharparkar cattle. The crossbred cattle showed 1082 differentially expressed genes (DEGs). Out of total DEGs, 597 genes were down-regulated and 485 were up-regulated. Their fold change varied from 2283.93 to −4816.02. Tharparkar cattle showed
875 differentially expressed genes including 451 down-regulated and 424 up-regulated. The fold change varied
from 94.93 to −19.20. A subset of genes was validated by qRT-PCR and results were correlated well with microarray data indicating that microarray results provided an accurate report of transcript level. Functional annotation study of DEGs confirmed their involvement in various pathways including response to oxidative stress,
immune system regulation, cell proliferation, cytoskeletal changes, kinases activity and apoptosis. Gene network
analysis of these DEGs plays an important role to understand the interaction among genes. It is therefore, hypothesized that the different susceptibility to tropical theileriosis exhibited by indigenous and crossbred cattle is due
to breed-specific differences in the dealing of infected cells with other immune cells, which ultimately influence
the immune response responded against T. annulata infection
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Date 2023-05-30T04:23:46Z
2023-05-30T04:23:46Z
2017-01-01
 
Type Article
 
Identifier Kumar, A., Gaur, G. K., Gandham, R. K., Panigrahi, M., Ghosh, S., Saravanan, B. C., ... & Sahoo, A. P. (2017). Global gene expression profile of peripheral blood mononuclear cells challenged with Theileria annulata in crossbred and indigenous cattle. Infection, Genetics and Evolution, 47, 9-18.
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/77874
 
Language English
 
Relation Not Available;
 
Publisher Not Available