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Plasmid-mediated antimicrobial resistance in motile aeromonads from diseased Nile tilapia (Oreochromis niloticus)

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Title Plasmid-mediated antimicrobial resistance in motile aeromonads from diseased Nile tilapia (Oreochromis niloticus)
Not Available
 
Creator Preena, P. G.,
Rejish Kumar, V. J.
Arathi Dharmaratnam
Swaminathan, T. R.
 
Subject Aeromonas
antibiotics
antimicrobial resistance
integrons
plasmid curing
 
Description We have isolated different aeromonads from diseased Nile tilapia and analysed the presence of virulent genes. For determining the exact location of ARG, plasmid profiling and plasmid curing followed by antibiotic susceptibility tests have been carried out for all the identified isolates.
For the sustainable farming of tilapia, proper maintenance of their health and ad equate treatment for infections at appropriate time are inevitable. The indiscriminate use of antibiotics in aquaculture, as a part of treatment and as growth promoters, accelerates antimicrobial resistance (AMR) among the fish pathogens. In the present study, we have isolated diverse aeromonads from Nile tilapia and studied their anti biogram and plasmid profiling. Aeromonas hydrophila, Aeromonas veronii, Aeromonas sobria, Aeromonas dhakensis, Aeromonas caviae, Aeromonas jandaei and Aeromonas aquatica were isolated from infected tilapia (n = 150), and their Shannon wiener di versity index was calculated as 1.926. A. veronii was found to be the most multiple antibiotic-resistant pathogen with the MAR index of 0.46, and A. aquatica was no ticed as the least resistant isolate. The minimum inhibitory concentration of resistant antibiotics was shown as >256 mcg/ml for most of the isolates. The virulent genes such as aerolysin and hemolysin were identified in all the isolates except A. aquatica. The detection of class 1 integrons, plasmid profiling and plasmid curing studies con firmed that AMR exhibited by most of the Aeromonas species is of plasmid mediated. This challenges the risk of wide spread of AMR among the pathogens and subsequent treatment of the infection.
National Surveillance Programme of Aquatic Animal Diseases, National Fisheries Development Board, Department of Animal Husbandry, Dairying and Fisheries (Grant Number: NFDB/Coord/NBFGR/2012-13/16720 dated 11.02.2013)
Science and Engineering Board (SERB), Department of Science and Technology for providing National Postdoctoral Research fellowship (PDF/2017/000378)
 
Date 2023-06-02T04:55:31Z
2023-06-02T04:55:31Z
2020-09-17
 
Type Research Paper
 
Identifier Preena, P. G., Arathi Dharmaratnam, Rejish Kumar, V. J., Swaminathan, T. R. 2021. Plasmid‐mediated antimicrobial resistance in motile aeromonads from diseased Nile tilapia (Oreochromis niloticus). Aquaculture Research, 52(1), 237-248. https://doi.org/10.1111/are.14886; First published: 17 September 2020; Published by Wiley Online Library.
1365-2109
http://krishi.icar.gov.in/jspui/handle/123456789/77978
 
Language English
 
Relation Not Available;
 
Publisher Wiley Online Library