Record Details

Conformational antigenic heterogeneity as a cause of the persistent fraction in HIV-1 neutralization.

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Conformational antigenic heterogeneity as a cause of the persistent fraction in HIV-1 neutralization.
 
Creator Colin, Phillippe
Ringe, Rajesh P.
Yasmeen, Anila
Ozorowski, Gabriel
Ketas, Thomas J.
Lee, Wen Hsin
Ward, Andrew B
Mooore, P John
Klasse, Per Johan
 
Subject QR Microbiology
 
Description Background Neutralizing antibodies (NAbs) protect against HIV-1 acquisition in animal models and show promise in treatment of infection. They act by binding to the viral envelope glycoprotein (Env), thereby blocking its receptor interactions and fusogenic function. The potency of neutralization is largely determined by affinity. Less well explained is the persistent fraction, the plateau of remaining infectivity at the highest antibody concentrations. Results We observed different persistent fractions for NAb neutralization of pseudovirus derived from two Tier-2 isolates of HIV-1, BG505 (Clade A) and B41 (Clade B): it was pronounced for B41 but not BG505 neutralization by NAb PGT151, directed to the interface between the outer and transmembrane subunits of Env, but negligible for either virus by NAb PGT145 to an apical epitope. Autologous neutralization by poly- and monoclonal NAbs from rabbits immunized with soluble native-like B41 trimer also left substantial persistent fractions. These NAbs largely target a cluster of epitopes in a hole in the dense glycan shield of Env around residue 289. We partially depleted B41-virion populations by incubating them with PGT145- or PGT151-conjugated beads. Each depletion reduced the sensitivity to the depleting NAb and enhanced it to the other. Autologous neutralization by the rabbit NAbs was reduced for PGT145-depleted and enhanced for PGT151-depleted B41 pseudovirus. Those changes in sensitivity encompassed both potency and the persistent fraction. We then compared soluble native-like BG505 and B41 Env trimers affinity-purified by one of three NAbs: 2G12, PGT145, or PGT151. Surface plasmon resonance showed differences among the fractions in antigenicity, including kinetics and stoichiometry, congruently with the differential neutralization. The large persistent fraction after PGT151 neutralization of B41 was attributable to low stoichiometry, which we explained structurally by the conformational plasticity of B41 Env. Conclusion Distinct antigenic forms even of clonal HIV-1 Env, detectable among soluble native-like trimer molecules, are distributed over virions and may profoundly mold neutralization of certain isolates by certain NAbs. Affinity purifications with some antibodies may yield immunogens that preferentially expose epitopes for broadly active NAbs, while shielding less cross-reactive ones. NAbs reactive with multiple conformers will together reduce the persistent fraction after passive and active immunization.
 
Publisher BMC
 
Date 2023-02-24
 
Type Article
PeerReviewed
 
Relation https://www.researchsquare.com/article/rs-2613503/v1
http://crdd.osdd.net/open/3080/
 
Identifier Colin, Phillippe and Ringe, Rajesh P. and Yasmeen, Anila and Ozorowski, Gabriel and Ketas, Thomas J. and Lee, Wen Hsin and Ward, Andrew B and Mooore, P John and Klasse, Per Johan (2023) Conformational antigenic heterogeneity as a cause of the persistent fraction in HIV-1 neutralization. RETROVIROLOGY.