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The physiological effect of rimI/rimJ silencing by CRISPR interference in Mycobacterium smegmatis mc2155

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title The physiological effect of rimI/rimJ silencing by CRISPR interference in Mycobacterium smegmatis mc2155
 
Creator Pal, Mohinder
Yadav , Kumar Vinay
Pal, Pramila
Agarwal, Nisheeth
Rao, Alka
 
Subject QR Microbiology
 
Description N-terminal acetylation of proteins is an important post-translational modification (PTM) found in eukaryotes and prokaryotes. In bacteria, N-terminal acetylation is suggested to play various regulatory roles related to protein stability, gene expression, stress response, and virulence; however, the mechanism of such response remains unclear. The proteins, namely RimI/RimJ, are involved in N-terminal acetylation in mycobacteria. In this study, we used CRISPR interference (CRISPRi) to silence rimI/rimJ in Mycobacterium smegmatis mc2155 to investigate the physiological effects of N-terminal acetylation in cell survival and stress response. Repeat analysis of growth curves in rich media and biofilm analysis in minimal media of various mutant strains and wild-type bacteria did not show significant differences that could be attributed to the rimI/rimJ silencing. However, total proteome and acetylome profiles varied significantly across mutants and wild-type strains, highlighting the role of RimI/RimJ in modulating levels of proprotein acetylation in the cellular milieu. Further, we observed a significant increase in the minimum inhibitory concentration (MIC) (from 64 to 1024 µg ml-1) for the drug isoniazid in rimI mutant strains. The increase in MIC value for the drug isoniazid in the mutant strains suggests the link between N-terminal acetylation and antibiotic resistance. The study highlights the utility of CRISPRi as a convenient tool to study the role of PTMs, such as acetylation in mycobacteria. It also identifies rimI/rimJ genes as necessary for managing cellular response against antibiotic stress. Further research would be required to decipher the potential of targeting acetylation to enhance the efficacy of existing antibiotics.
 
Publisher Springer Science
 
Date 2023-04-29
 
Type Article
PeerReviewed
 
Relation https://https://link.springer.com/article/10.1007/s00203-023-03561-5
http://crdd.osdd.net/open/3099/
 
Identifier Pal, Mohinder and Yadav , Kumar Vinay and Pal, Pramila and Agarwal, Nisheeth and Rao, Alka (2023) The physiological effect of rimI/rimJ silencing by CRISPR interference in Mycobacterium smegmatis mc2155. Archives of microbiology, 205 (5). ISSN 0302-8933