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Transcriptome profiling reveals the expression and regulation of genes associated with Fusarium wilt resistance in chickpea (Cicer arietinum L.)

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Relation http://oar.icrisat.org/12108/
https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/tpg2.20340
https://doi.org/10.1002/tpg2.20340
 
Title Transcriptome profiling reveals the expression and regulation of genes associated with Fusarium wilt resistance in chickpea
(Cicer arietinum L.)
 
Creator Garg, V
Chitikineni, A
Sharma, M
Ghosh, R
Samineni, S
Varshney, R K
Kudapa, H
 
Subject Chickpea
 
Description Fusarium wilt (FW) is one of the most significant biotic stresses limiting chickpea production worldwide. To dissect the molecular mechanism of FW resistance in chickpea, comparative transcriptome analyses of contrasting resistance sources of chickpea genotypes under control and Fusarium oxysporum f. sp. ciceris (Foc) inoculated conditions were performed. The high-throughput transcriptome sequencing
generated about 1137 million sequencing reads from 24 samples representing two resistant genotypes, two susceptible genotypes, and two near-isogenic lines under
control and stress conditions at two-time points (7th- and 12th-day post-inoculation). The analysis identified 5182 differentially expressed genes (DEGs) between different
combinations of chickpea genotypes. Functional annotation of these genes indicated their involvement in various biological processes such as defense response, cell wall biogenesis, secondary metabolism, and disease resistance. A significant number (382) of transcription factor encoding genes exhibited differential expression patterns under stress. Further, a considerable number of the identified DEGs (287) co-localized with previously reported quantitative trait locus for FWresistance. Several resistance/susceptibility-related genes, such as SERINE/THREONINE PROTEIN KINASE, DIRIGENT, and MLO exhibiting contrasting expression patterns in resistant and susceptible genotypes upon Foc inoculation, were identified. The results presented in the study provide valuable insights into the transcriptional dynamics associated with FW stress response in chickpea and provide candidate genes for the
development of disease-resistant chickpea cultivars.
 
Publisher Crop Science Society of America
 
Date 2023-03-27
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/12108/1/The%20Plant%20Genome_01_16_2023.pdf
Garg, V and Chitikineni, A and Sharma, M and Ghosh, R and Samineni, S and Varshney, R K and Kudapa, H (2023) Transcriptome profiling reveals the expression and regulation of genes associated with Fusarium wilt resistance in chickpea (Cicer arietinum L.). The Plant Genome (TSI). 01-16. ISSN 1940-3372