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A comparative study of xylanase producing wild and mutated strains of Streptomyces variabilis VITMUVB02 isolated from Kanyakumari salt pan

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Title A comparative study of xylanase producing wild and mutated strains of Streptomyces variabilis VITMUVB02 isolated from Kanyakumari salt pan
 
Creator Bhattacharya, U
Manvi, S
Sreedharan, V
Rao, K V B
 
Subject Actinobacteria
Birchwood
Physical mutation
Streptomyces variabilis
Xylanase
 
Description 822-831
The current study aimed to isolate xylanase-producing marine actinobacteria, which is a novel idea; ascribed to the
challenges associated with the culturing of marine microorganisms. A total of 5 actinobacterial colonies were isolated from
Kanyakumari marine sediments. Among them only one isolate, A1 indicated the elevated xylanase activity manifesting a
zone of hydrolysis of around 20 mm during primary screening. On the other hand, secondary screening of A1 i.e., sugar
estimation by 3,5-dinitrosalicylic acid method, resulted in A1 exhibiting 400 ìg/ml of enzyme activity. The enzyme
extracted from the isolate A1 also contained a protein concentration of 600 ìg/ml. Physical and chemical mutation studies
were carried out to over-express xylanase production. Chemical mutation involving the use of EDTA did not show a
significant increase in xylanase production and thus, was not subjected to further analyses.
The isolate, A1 also showed a zone of hydrolysis of 28 mm in the physical mutation study and was named A'1.75. This zone
was 8 mm more than the wild-type strain after UV-exposure for 75 min. A'1.75 also exhibited an enzyme activity of 600 ìg/ml
and protein content of 800 ìg/ml. The potential isolate was identified as Streptomyces variabilis VITMUVB02 using 16S rRNA
molecular sequencing. The molecular weight of the purified xylanase extracted from A1 and A'1.75 was found to be 25 kDa
and 20 kDa, respectively. Fourier Transform Infrared spectroscopy (FT-IR) and High-Performance Liquid Chromatography
(HPLC) analyses showed the mutagenic effect with the change in the spectral and functional groups.
 
Date 2023-06-27T11:59:19Z
2023-06-27T11:59:19Z
2023-06
 
Type Article
 
Identifier 2582-6727 (Online); 2582-6506 (Print)
http://nopr.niscpr.res.in/handle/123456789/62120
https://doi.org/10.56042/ijms.v51i10.2931
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJMS Vol.51(10) [October 2022]