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Anticancer potential of methanol extract of seeds of Artocarpus hirsutus in human breast cancer cell lines

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Title Anticancer potential of methanol extract of seeds of Artocarpus hirsutus in human breast cancer cell lines
 
Creator Shrirang, Hogale Mangesh
Kariyil, Bibu John
John, Reni
 
Subject Artocarpus hirsutus
Apoptosis
Bcl-2
GC-HRMS
Intrinsic pathway
MTT assay
 
Description 390-400
Artocarpus hirsutus, belonging to the family Moraceae, is used traditionally for the treatment of skin
problems, diarrhoea, snake bite and ulcers. In the present study, the methanol extracts of fruit seeds of
A. hirsutus (MAH) (Wild jack) were investigated for the presence of phytochemicals and anticancer activities
against human breast cancer cell lines in vitro. Methanolic extraction was done using soxhlet. The phytochemicals
were determined by conventional qualitative tests and GC-HRMS. Cytotoxicity study was conducted using
MTT assay. Apoptotic changes produced by the plant extract in the treated cells were determined by acridine
orange ethidium bromide and hoechst staining. Apoptotic pathway was analysed using JC-1 staining and molecular
expressions of Bcl-2. Protein and gene expressions of Bcl-2 were studied by performing western blot and
RT-qPCR respectively. In the cytotoxicity study, MAH exhibited significant (p
 
Date 2023-06-28T11:46:13Z
2023-06-28T11:46:13Z
2023-06
 
Type Article
 
Identifier 0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscpr.res.in/handle/123456789/62174
https://doi.org/10.56042/ijtk.v22i2.34984
 
Language en
 
Relation Int Cl.23: A61K 36/00, A61K 45/00
 
Publisher NIScPR-CSIR,India
 
Source IJTK Vol.22(2) [April 2023]