Anticancer potential of methanol extract of seeds of Artocarpus hirsutus in human breast cancer cell lines
NOPR - NISCAIR Online Periodicals Repository
View Archive InfoField | Value | |
Title |
Anticancer potential of methanol extract of seeds of Artocarpus hirsutus in human breast cancer cell lines
|
|
Creator |
Shrirang, Hogale Mangesh
Kariyil, Bibu John John, Reni |
|
Subject |
Artocarpus hirsutus
Apoptosis Bcl-2 GC-HRMS Intrinsic pathway MTT assay |
|
Description |
390-400
Artocarpus hirsutus, belonging to the family Moraceae, is used traditionally for the treatment of skin problems, diarrhoea, snake bite and ulcers. In the present study, the methanol extracts of fruit seeds of A. hirsutus (MAH) (Wild jack) were investigated for the presence of phytochemicals and anticancer activities against human breast cancer cell lines in vitro. Methanolic extraction was done using soxhlet. The phytochemicals were determined by conventional qualitative tests and GC-HRMS. Cytotoxicity study was conducted using MTT assay. Apoptotic changes produced by the plant extract in the treated cells were determined by acridine orange ethidium bromide and hoechst staining. Apoptotic pathway was analysed using JC-1 staining and molecular expressions of Bcl-2. Protein and gene expressions of Bcl-2 were studied by performing western blot and RT-qPCR respectively. In the cytotoxicity study, MAH exhibited significant (p |
|
Date |
2023-06-28T11:46:13Z
2023-06-28T11:46:13Z 2023-06 |
|
Type |
Article
|
|
Identifier |
0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscpr.res.in/handle/123456789/62174 https://doi.org/10.56042/ijtk.v22i2.34984 |
|
Language |
en
|
|
Relation |
Int Cl.23: A61K 36/00, A61K 45/00
|
|
Publisher |
NIScPR-CSIR,India
|
|
Source |
IJTK Vol.22(2) [April 2023]
|
|