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Transcriptional reprogramming under vancomycin pressure in Staphylococcus aureus

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Title Transcriptional reprogramming under vancomycin pressure in Staphylococcus aureus
 
Creator Ghosh, Prerona
Kumar, Aakash
Mohapatra, Dharitri
Mohapatra, Saroj Kant
 
Subject Heteroresistance
Peptidoglycan
 
Description 534-545
The World Health Organization (WHO) has defined Vancomycin intermediate Staphylococcus aureus (VISA) and
Vancomycin resistant Staphylococcus aureus (VRSA) as high priority pathogens. VISA and VRSA are produced by
different mechanisms, hence VISA cannot convert to VRSA. Consequently, upon vancomycin treatment of VSSA isolates,
there can be emergence of VISA but not its conversion to VRSA. But we observed that when VSSA (MIC ≤2 μg/mL)
isolates, lacking vanA or vanB genes, are grown under vancomycin stress for 60 days, 4 out of 8 isolates get converted to
VISA (MIC = 4-8 μg/mL), and 1 converted to VRSA (MIC ≥16 μg/mL). Further, the VRSA isolate had a vancomycin MIC
that was 8-fold higher than that of its sensitive counterpart. Hence, the VRSA has been interpreted as a heteroresistant
isolate. To dissect the molecular underpinning of this transient resistance pattern, we analyzed the gene expression profile of
these isolates and publicly available datasets. pbp2 gene was observed to be consistently upregulated in all the VISA isolates
except the heteroresistant isolate. Pathway analysis revealed upregulation of peptidoglycan biosynthesis in VISA isolates.
However, the distinct transcriptional profile of the heteroresistant isolate (with upregulation of recR, ureC and atl) suggests
potential role of increased mutation due to SOS response or biofilm formation in this phenotype.
 
Date 2023-06-30T05:38:32Z
2023-06-30T05:38:32Z
2023-06
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/62213
https://doi.org/10.56042/ijeb.v61i07.2211
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.61(07) [July 2023]