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Apoptotic‑like PCD inducing HRC gene when silenced enhances multiple disease resistance in plants

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Relation http://oar.icrisat.org/12126/
https://www.nature.com/articles/s41598-022-24831-0
https://doi.org/10.1038/s41598-022-24831-0
 
Title Apoptotic‑like PCD inducing HRC gene when silenced enhances multiple disease resistance in plants
 
Creator Kushalappa, A C
Hegde, N G
Gunnaiah, R
Sathe, A
Yogendra, K N
Ajjamada, L
 
Subject Plant Genetics
Plant Disease
 
Description Programmed cell death (PCD) plays an important role in plant environmental stress and has the potential to be manipulated to enhance disease resistance. Plants have innate immunity and, following pathogen perception, the host induces a Hypersensitive Response PCD (HR-PCD), leading to pattern (PTI) or effector triggered immunity (ETI). Here we report a non-HR type or Apoptotic-Like PCD (AL-PCD) in pathogen infected wheat and potato based on apoptotic-like DNA fragmentation. A deletion mutation in the gene encoding histidine rich calcium binding protein (TaHRC) in FHB-resistant wheat (R-NIL) failed to induce AL-PCD. Similarly, the CRISPR-Cas9 based silencing of StHRC gene in Russet Burbank potato failed to induce apoptotic-like DNA fragmentation, proved based on DNA laddering and TUNEL assays. The absence of AL-PCD in wheat R-NIL reduced pathogen biomass and mycotoxins, increasing the accumulation of resistance metabolites and FHB-resistance, and in potato
it enhanced resistance to multiple pathogens. In addition, the reduced expressions of metacaspase (StMC7) and Ca2+
dependent endonuclease 2 (StCaN2) genes in potato with Sthrc indicated an involvement of a hierarchy of genes in the induction of AL-PCD. The HRC in commercial varieties of
different crops, if functional, can be silenced by genome editing possibly to enhance resistance to multiple pathogens.
 
Publisher Nature Publishing Group
 
Date 2022-11-27
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights cc_attribution
 
Identifier http://oar.icrisat.org/12126/1/Scientific%20Reports_12_01-13_2022.pdf
Kushalappa, A C and Hegde, N G and Gunnaiah, R and Sathe, A and Yogendra, K N and Ajjamada, L (2022) Apoptotic‑like PCD inducing HRC gene when silenced enhances multiple disease resistance in plants. Scientific Reports (TSI), 12. 01-13. ISSN 2045-2322