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Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida)

Indian Agricultural Research Journals

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Title Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida)
 
Creator USHA, USHA
JANAKIRAM, T
PRASAD, K V
KAUR, CHARANJIT
SRINIVASAN, P
SATYAVATHI, C TARA
 
Subject Anthocyanin, Callus cultures, Colour value index, Fructose, Galactose, Glucose, Petunia hybrida, Sucrose
 
Description Anthocyanins are naturally occurring compounds that impart color to fruits, flowers, vegetables, and plants. They are probably the most important group of visible plant pigments besides chlorophyll pigments. Apart from imparting color to plants, anthocyanins also have an array of health-promoting benefits, as they can protect against a variety of free radicals through a various number of mechanisms. Development of an efficient tissue culture system for commercial production of anthocyanins requires an integrated approach through manipulation of various media constituents. The effect of varied concentrations of different carbon sources on anthocyanin production in callus cultures of Petunia hybrida cv Bravo Blue was studied. Explants from young leaves were cultured on Murashige and Skoog (MS)medium supplemented with MS + IBA (19.6 μM) + Kin. (4.65 μM) + AdS (81.45 mM), 3% sucrose and 0.7% agar. Among the various carbon sources tested, incorporation of Glucose at 5% was found to have earliest pigment induction with maximum response coefficient with highest pigment content (1.36 ± 0.012 CV/g FCW). Highest gain in fresh cell weight was noticed with the addition of sucrose 5% (3.96 ± 0.06 g). When MS medium was supplemented with different concentrations of Galactose, the explants failed to respond.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2014-08-11
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/43085
 
Source The Indian Journal of Agricultural Sciences; Vol 84, No 8 (2014)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/43085/19132
 
Rights Copyright (c) 2014 The Indian Journal of Agricultural Sciences