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Molecular characterization of phycobiliproteins producing Cyanobacteria by using nanopore sequencer isolated from dairy effluent

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Title Molecular characterization of phycobiliproteins producing Cyanobacteria by using nanopore sequencer isolated from dairy effluent
 
Creator Kabariya, Jayesh H
Ramani, Vimal M
 
Subject 16S rRNA sequencing
Dairy effluent treatment
Oscillatoria species
 
Description 423-429
Cyanobacterial taxonomy has been established based on morphological features, such as the shape and dimensions of the
cells, and the presence of structurally differentiated cells. Since, the last decade's molecular techniques were used for the
identification of cyanobacteria based on 16S rRNA genes. Nanopore sequencing technology is the most recent technique so,
in the present study nanopore sequencing was explored for the identification of cyanobacterial species isolated from dairy
effluent and screened based on effluent nutrients removal efficiency and it also phycobiliproteins producer. The steps were
followed by DNA extraction, library preparation, barcoding, DNA amplification, 16S rRNA sequencing by using MinION
Nanopore sequencer and data analysis was evaluated by Epi2Me software for cyanobacterial species identification and
preparation of the phylogeny. The two cyanobacterial species (Oscillatoria species-1 & Oscillatoria species-2) were
molecularly identified as Oscillatoria acuminata and Oscillatoria sancta by 16S rRNA sequencing using Nanopore
sequencing Technology and the DNA sequence was submitted to NCBI with stain name VMRJHK2020 and
VMRJHK012020 and the Gene bank accession are MW364270 and MW364271, respectively.
 
Date 2023-07-20T06:19:33Z
2023-07-20T06:19:33Z
2023-07
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62302
https://doi.org/10.56042/ijbb.v60i5.1319
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.60(05) [May 2023]