Molecular characterization of phycobiliproteins producing Cyanobacteria by using nanopore sequencer isolated from dairy effluent
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Title |
Molecular characterization of phycobiliproteins producing Cyanobacteria by using nanopore sequencer isolated from dairy effluent
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Creator |
Kabariya, Jayesh H
Ramani, Vimal M |
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Subject |
16S rRNA sequencing
Dairy effluent treatment Oscillatoria species |
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Description |
423-429
Cyanobacterial taxonomy has been established based on morphological features, such as the shape and dimensions of the cells, and the presence of structurally differentiated cells. Since, the last decade's molecular techniques were used for the identification of cyanobacteria based on 16S rRNA genes. Nanopore sequencing technology is the most recent technique so, in the present study nanopore sequencing was explored for the identification of cyanobacterial species isolated from dairy effluent and screened based on effluent nutrients removal efficiency and it also phycobiliproteins producer. The steps were followed by DNA extraction, library preparation, barcoding, DNA amplification, 16S rRNA sequencing by using MinION Nanopore sequencer and data analysis was evaluated by Epi2Me software for cyanobacterial species identification and preparation of the phylogeny. The two cyanobacterial species (Oscillatoria species-1 & Oscillatoria species-2) were molecularly identified as Oscillatoria acuminata and Oscillatoria sancta by 16S rRNA sequencing using Nanopore sequencing Technology and the DNA sequence was submitted to NCBI with stain name VMRJHK2020 and VMRJHK012020 and the Gene bank accession are MW364270 and MW364271, respectively. |
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Date |
2023-07-20T06:19:33Z
2023-07-20T06:19:33Z 2023-07 |
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Type |
Article
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Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62302 https://doi.org/10.56042/ijbb.v60i5.1319 |
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Language |
en
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Publisher |
NIScPR-CSIR,India
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Source |
IJBB Vol.60(05) [May 2023]
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