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Effects of Morpholino-mediated knockdown of the Angiotensin-converting enzyme 2 (ACE-2) on dopamine Neurogenesis and the Hypothalamic Pituitaryadrenal (HPA) Axis in Larval Zebrafish (Danio rerio)

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Title Effects of Morpholino-mediated knockdown of the Angiotensin-converting enzyme 2 (ACE-2) on dopamine Neurogenesis and the Hypothalamic Pituitaryadrenal (HPA) Axis in Larval Zebrafish (Danio rerio)
 
Creator Christy, L Divya
Raza, Mir Abbas
AC, Clarita
Nellore, Jayshree
 
Subject ACE2 gene
Dopamine neurogenesis
HPA axis
Zebrafish development
COVID-19 and neurological manifestations
 
Description 414-422
While the presence of ACE2 and its expression in brain structures, along with its recent link with the novel SARS-CoV-2,
are known, their impact on the development of the brain and on the activity of the HPA axis in larval zebrafish is not known.
Therefore, the primary objective of this study was to determine how silencing the ACE2 gene affects DA neurogenesis and
the endocrine stress response in larval zebrafish (Danio rerio). In this investigation, an ACE2 AUG morpholino
oligonucleotide was introduced by electroporation into the 96 hpf zebrafish in order to silence the expression of the ACE2
gene using antisense technology. Electroporation of the embryo in 1X PBS on 1.5% Agarose gel with ACE2 AUG
morpholino oligonucleotide was done to transfer it into the embryo. After successful transfer, no visible morphological
changes were observed. A significant reduction in ACE2 gene expression confirms the successful oligonucleotide transfer.
Nurr1 expression was significantly reduced as ACE2 expression was silenced, but there was no significant effect on GCR or
CRH expression. Interestingly, a significant increase in locomotor activity was seen after the silencing of ACE2 expression.
Overall, these results facilitate the prediction of possible neurological manifestations of brain ACE2 dysregulation during
and after COVID-19 infection.
 
Date 2023-07-20T06:22:01Z
2023-07-20T06:22:01Z
2023-07
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62303
https://doi.org/10.56042/ijbb.v60i5.477
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.60(05) [May 2023]