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The role of expressed T-cells cytokines mRNAs from endometrial tissue in patients with unexplained infertility

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Title The role of expressed T-cells cytokines mRNAs from endometrial tissue in patients with unexplained infertility
 
Creator Ciraci, Enver
Sahin, Sadik
Herkiloglu, Emine Dilsad
Ahmad, Sarfraz
Unal, Tayfun
Tetik, Sermin
 
Subject Immunodulatories
mRNA
q-PCR
Therapeutic targets
Unexplained infertility
 
Description 406-413
The aim of this study was to investigate the immunomodulatory process and cytokines parameters that potentially
modulates unexplained infertility (UI) by monitoring changes in the mRNA expression levels. From 52 UI patients who
underwent biopsy, we collected endometrial tissue specimens between 21st-24th days of the menstrual cycles. Specimens
were also collected from 34 matched healthy-fertility women, which served as control. Blood hormone levels were
evaluated on 2nd and 3rd day of the menstrual cycles. We measured mRNAs of pro-inflammatory cytokines [tumor necrosis
factor-alpha (TNF-α), interleukin (IL)-8], anti-inflammatory cytokine [IL-10], and other cytokines [IL-12A, IL-17A, IL-20,
leukocyte inhibitory factor (LIF), transforming growth factor-β (TGF-β)]. Real-time q-PCR was used to examine
immunomodulatory effects on UI cases. There was no significant difference between UI and control groups in terms of age,
body-mass index (BMI), follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin, thyroid-stimulating
hormone (TSH), free T4 (FT4), and progesterone levels (P> 0.05). Estradiol (E2) levels were significantly different between
the groups (P< 0.01). Compared to fertile-controls, mRNA expression levels of IL-12A, IL-17A, TNF-α, and LIF were
significantly lower in UI specimens (P< 0.05). IL-10 mRNA expressions were higher in UI than fertile-controls (P< 0.05).
Spearman test demosrated positive correlation between cytokines in the UI group (P< 0.05). Our findings highlight
that depending on the patients’ immunity, decreased expressions of IL-12A, IL-17A, LIF, TNF-α, and higher expressions
of IL-10 in the endometrium of UI patients may be one of the potential cellular/molecular alteration mechanisms of
infertility. Better understanding of these complex mechanisms may provide newer therapeutic targets for managing UI.
 
Date 2023-07-20T06:24:40Z
2023-07-20T06:24:40Z
2023-07
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62304
https://doi.org/10.56042/ijbb.v60i5.1224
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.60(05) [May 2023]