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Mutations in the ubiquitin gene of Saccharomyces cerevisiae accompanied by divergent use of CUG codon affect morphogenesis in Candida albicans

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Title Mutations in the ubiquitin gene of Saccharomyces cerevisiae accompanied by divergent use of CUG codon affect morphogenesis in Candida albicans
 
Creator Doshi, Ankita
Dantuslia, Sandeep Kumar
Prabha, C Ratna
 
Subject Candida albicans
Hyphae
Morphogenesis
Ubiquitin mutations
Yeast form
 
Description 516-524
UbEP42, a mutant of ubiquitin constitutes four mutations, namely S20F, A46S, L50P, and I61T. Characterization of UbEP42 and the isolated mutations revealed that UbEP42, UbL50P, and UbI61T conferred dosage-dependent lethality on S.cerevisiae, while UbS20F and UbA46S produced no effect over survival. In the present study, opportunistic humanpathogen Candida albicans has been employed here to investigate the influence of ubiquitin mutations of S. cerevisiae onmorphogenesis, besides various ubiquitin-dependent functions. The codon for leucine ‘CUG’ in S. cerevisiae is read asserine in C. albicans. Hence the protein variants expressed from the S. cerevisiae gene are added with the prefix ‘Sc’.ScUbEP42, ScUbL50P, and ScUbI61T had a negative influence over protein trafficking, lysosomal degradation of proteins,and polyubiquitination with K48 and K63 linkages in C. albicans as in S. cerevisiae. ScUbEP42 and all four derivativemutations impaired the morphogenesis of the yeast form into infective hyphal form. The impairment of morphogenesis ofC.albicans by ubiquitin mutations is unprecedented and suggests a target pathway for future therapeutics.
 
Date 2023-08-03T07:16:09Z
2023-08-03T07:16:09Z
2023-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62402
https://doi.org/10.56042/ijbb.v60i7.4265
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.60(07) [July 2023]