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Clitoria ternatea extract-loaded chitosan nanoparticles ameliorate diabetes and oxidative stress in diabetic rats

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Title Clitoria ternatea extract-loaded chitosan nanoparticles ameliorate diabetes and oxidative stress in diabetic rats
 
Creator Mobasher, Maysa A
Baioumy, Shereen A
Alazzouni, Ahmed S
Khayyat, Arwa Ishaq A
Awad, Nabil S
Abdel-Hakeem, Mohamed A
Al-Sowayan, Noorah Saleh
 
Subject Antioxidant Markers
Liver enzymes
Diabetes mellitus
Nano-drug delivery
Gene expression
Streptozotocin
 
Description 501-515
Type 2 diabetes mellitus (T2DM) is one of the expanding global health problems and is the most common metabolic
disorder characterized by hyperglycemia, which significantly contributes to producing reactive oxygen species (ROS). More
than 400 plant species with hypoglycemic activity have been mentioned in the literature. Clitoria ternatea (C. ternatea), often
called Butterfly pea or Asian pigeonwing, is a plant species member of the Fabaceae family. The main goal of this study was to
evaluate the methanolic extract of C. ternatea (CT-Mx)'s and/or chitosan-loaded nanoparticles (CHNPs) antihyperglycemic and
antioxidant effects in normal and diabetic rats produced by streptozotocin (STZ). A total of 20 male albino rats had been
divided into 4 groups, control non-diabetic (NC), STZ/diabetic control, STZ/diabetic + CT-Mx, and STZ/diabetic + CT-CHNPs
groups. After 28 days, levels of insulin, fasting blood glucose (FBG), aspartate transaminase (AST), alanine transaminase
(ALT), superoxide dismutase (SOD), glutathione (GSH), lipid peroxidation, and mRNA gene expression were assessed.
Histopathological and immunohistochemical studies were performed for pancreatic tissues. In the STZ/diabetic (Gp2) rats,
levels of FBG, AST, ALT, and both CDKN1A and TP53 gene expression were significantly increased. Moreover, the
hyperglycemia-induced hepatic oxidative state is evidenced by a significant increment of lipid peroxidation and deterioration in
SOD and GSH levels. On the contrary, both the STZ/diabetic + CT-Mx and STZ/diabetic + CT-CHNPs showed discernible
improvement in diabetes-associated complications; however, STZ/diabetic + CT-CHNPs (Gp4) rats significantly suppressed the
generated oxidative stress and improved antioxidant activity, liver function, and insulin secretion. Also, their pancreatic section
exhibited architecture with normal regenerative pancreatic endocrine islets with normal distribution and number of beta cells
and suppressing inflammatory and apoptotic gene expression compared to Gp2. Nanocarrier agents showed excellent
antihyperglycemic and effects after antioxidative, making it a promising technology for diabetics.
 
Date 2023-08-03T07:19:27Z
2023-08-03T07:19:27Z
2023-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62403
https://doi.org/10.56042/ijbb.v60i7.4140
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.60(07) [July 2023]