Clitoria ternatea extract-loaded chitosan nanoparticles ameliorate diabetes and oxidative stress in diabetic rats
NOPR - NISCAIR Online Periodicals Repository
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Title |
Clitoria ternatea extract-loaded chitosan nanoparticles ameliorate diabetes and oxidative stress in diabetic rats
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Creator |
Mobasher, Maysa A
Baioumy, Shereen A Alazzouni, Ahmed S Khayyat, Arwa Ishaq A Awad, Nabil S Abdel-Hakeem, Mohamed A Al-Sowayan, Noorah Saleh |
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Subject |
Antioxidant Markers
Liver enzymes Diabetes mellitus Nano-drug delivery Gene expression Streptozotocin |
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Description |
501-515
Type 2 diabetes mellitus (T2DM) is one of the expanding global health problems and is the most common metabolic disorder characterized by hyperglycemia, which significantly contributes to producing reactive oxygen species (ROS). More than 400 plant species with hypoglycemic activity have been mentioned in the literature. Clitoria ternatea (C. ternatea), often called Butterfly pea or Asian pigeonwing, is a plant species member of the Fabaceae family. The main goal of this study was to evaluate the methanolic extract of C. ternatea (CT-Mx)'s and/or chitosan-loaded nanoparticles (CHNPs) antihyperglycemic and antioxidant effects in normal and diabetic rats produced by streptozotocin (STZ). A total of 20 male albino rats had been divided into 4 groups, control non-diabetic (NC), STZ/diabetic control, STZ/diabetic + CT-Mx, and STZ/diabetic + CT-CHNPs groups. After 28 days, levels of insulin, fasting blood glucose (FBG), aspartate transaminase (AST), alanine transaminase (ALT), superoxide dismutase (SOD), glutathione (GSH), lipid peroxidation, and mRNA gene expression were assessed. Histopathological and immunohistochemical studies were performed for pancreatic tissues. In the STZ/diabetic (Gp2) rats, levels of FBG, AST, ALT, and both CDKN1A and TP53 gene expression were significantly increased. Moreover, the hyperglycemia-induced hepatic oxidative state is evidenced by a significant increment of lipid peroxidation and deterioration in SOD and GSH levels. On the contrary, both the STZ/diabetic + CT-Mx and STZ/diabetic + CT-CHNPs showed discernible improvement in diabetes-associated complications; however, STZ/diabetic + CT-CHNPs (Gp4) rats significantly suppressed the generated oxidative stress and improved antioxidant activity, liver function, and insulin secretion. Also, their pancreatic section exhibited architecture with normal regenerative pancreatic endocrine islets with normal distribution and number of beta cells and suppressing inflammatory and apoptotic gene expression compared to Gp2. Nanocarrier agents showed excellent antihyperglycemic and effects after antioxidative, making it a promising technology for diabetics. |
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Date |
2023-08-03T07:19:27Z
2023-08-03T07:19:27Z 2023-08 |
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Type |
Article
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Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62403 https://doi.org/10.56042/ijbb.v60i7.4140 |
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Language |
en
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Publisher |
NIScPR-CSIR,India
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Source |
IJBB Vol.60(07) [July 2023]
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