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Prokaryotic and tissue-specific expression of RglKAT gene from Rehmannia glutinosa

Indian Agricultural Research Journals

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Title Prokaryotic and tissue-specific expression of RglKAT gene from Rehmannia glutinosa
 
Creator ZHOU, Y Q
ZHANG, Y H
WANG, W S
WANG, X N
DUAN, H Y
 
Subject Cloning, Expression, 3-Ketoacyl-CoA thiolase (KAT), Rehmannia glutinosa, Real Time quantitative PCR
 
Description A 1640-bp full-length cDNA of the 3-ketoacyl-CoA thiolase gene was cloned from Rehmannia glutinosa L. leaf using RT-PCR and RACE. Bioinformatics analyses indicated that it contained one 1395-bp open reading frameencoding a thiolase of 464 amino acids with the predicted molecular weight of 49 kDa, and had a similarity of 73-82% to other species at the nucleotide level and 63-88% to other species at the amino acid level, respectively. The recombinant RglKAT was expressed in a bacterial system in an active form, analyzed by PAGE-CBB staining and purified using Ni-agarose chromatography. Real Time quantitative PCR analysis showed that relative expression levels of the RglKATgene differed between R.glutinosa tissues, with the highest levels in the petals and the lowest in the early senescing stems. These results revealed that RglKAT gene could be cloned and heterogeneously expressed, and related to R. glutinosa growth and development.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2015-11-19
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/53762
 
Source The Indian Journal of Agricultural Sciences; Vol 85, No 11 (2015)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/53762/22778
 
Rights Copyright (c) 2015 The Indian Journal of Agricultural Sciences