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Upregulation of Psoriasinin Cholesteatoma associated with inflammation, bone destruction and severity of disease: Histo-cyto-chemical and ultrastructural studies

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Title Upregulation of Psoriasinin Cholesteatoma associated with inflammation, bone destruction and severity of disease: Histo-cyto-chemical and ultrastructural studies
 
Creator Kairo, Arvind Kumar
Sarwar, Saba
Quadri, Javed Ahsan
Singh, Anup
Kumar, Rakesh
Thakar, Alok
Sharma, Suresh Chandra
Shariff, Ahmadulla
 
Subject Bacterial localization
Cholesteatoma
Histo-cyto-chemistry
Psoriasin
Tympanomastoidectomy
Ultrastructure
 
Description 624-631
Cholesteatoma remains a mystery and the factors triggering and propagating it unknown. This study was aimed to evaluate the role of Psoriasin and pro-inflammatory cytokines in progression of middle ear cholesteatoma. In this study 18 patients (12 cases of cholesteatoma and six cases of posterosuperior retraction pocket (PSRP) without cholesteatoma) were included in the study. Clinical aggressiveness was evaluated on-table under operative microscope by evaluating erosion of ossicles, invasion of surrounding structures. The classification of disease was also based on the radiological (CT-scan) findings. Based on clinical aggressiveness, the cholesteatoma cases were divided into mild, moderate, and severe. The tissues were processed for histopathological, ultrastructural and immunohistochemical analysis. The Psoriasin expression in cholesteatoma tissues was significantly higher (P< 0.05) compared to the PSRP tissue and increased with the increasing severity of the disease. The pro-inflammatory cytokines (IL-6 and IL-1β) were also upregulated along with vascular endothelial growth factor and Matrix metalloproteinase-13 in cholesteatoma cases compared to control. Transmission Electron Microscopic images of ultra-thin sections of the tissues showed numerous secretory vesicles, most likely containing cytokines and a unique ultrastructural feature of bacterial localization within the membrane pocket was seen, which gave direct evidence of bacterial infection in the middle ear cholesteatoma.
 
Date 2023-08-14T09:26:46Z
2023-08-14T09:26:46Z
2023-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62417
https://doi.org/10.56042/ijbb.v60i8.4299
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.60(08) [August 2023]