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In vitro larvicidal, antioxidant, and DNA binding activity of biosynthesized zinc oxide nanoparticles conjugated with Andrographis paniculata aqueous extract

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Title In vitro larvicidal, antioxidant, and DNA binding activity of biosynthesized zinc oxide nanoparticles conjugated with Andrographis paniculata aqueous extract
 
Creator Dwivedi, Manish Kumar
Singh, Prashant Kumar
 
Subject Anopheles culicifacies
Andrographis paniculata
Antioxidant activity
Larvicidal
Nanoparticles
 
Description 611-623
Anopheles culicifacies is the major vector responsible for the transmission of human malarial parasite Plasmodium sp.
The use of green synthesized nanoparticles has emerged as an interesting advancement for the development of
plant-mediated insecticides that can be effective against widespread insecticide resistance. In the current study, we report on
the larvicidal, antioxidant, H2O2 sensitivity, and hemolytic properties of green synthesized zinc oxide nanoparticles using
Andrographis paniculata (ZnO-APNPs). The ZnO-APNPs were characterized using UV-Visible spectroscopy, FTIR, XRD,
and TEM analysis. Crystalline and spherical NPs with an average particle size found to be ~17 nm were synthesized. The
UV absorption spectra of the CT-DNA interaction with ZnO-APNPs revealed a bathochromic effect indicating groove
binding. The ZnO-APNPs displayed lethality against A. culicifacies larvae after 24 h and 48 h of exposure. The LC50 and
LC90values were 31.75±2.23 ppm and 189.56±2.44 ppm and 95.45±1.56 ppm, 432.36±1.34 ppm at different concentrations
respectively. ZnO-APNPs offered complete larval mortality within 24 h and 48 h of exposure. Strong concentrationdependent
antioxidant property with a free radical inhibition of 39.51±1.25 % was observed while the ZnO-APNPs were
found to be non toxic in hemolysis assay. The strong potency of ZnO-APNPs in killing A. culicifacies larvae advocates their
further development as potential natural, reliable and ecofriendly larvicides.
 
Date 2023-08-14T09:29:42Z
2023-08-14T09:29:42Z
2023-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62418
https://doi.org/10.56042/ijbb.v60i8.4295
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.60(08) [August 2023]