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Urinary exosome isolation in fluorosis patients with early symptoms of nephrotoxicity

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Title Urinary exosome isolation in fluorosis patients with early symptoms of nephrotoxicity
 
Creator Sarwar, Saba
Quadri, Javed Ahsan
Shariff, Ahmadulla
 
Subject Atomic Force Microscopy (AFM)
Exosomes
Fluorosis
Proteinuria
Renal impairments
Transmission Electron Microscopy (TEM)
 
Description 596-600
Polyureic nephropathy, a kidney disease characterized by excess thirst & frequent urination, are established
manifestations of non-skeletal fluorosis. This study aimed to isolate the urinary exosomes from fluorosis patients with early
renal impairment. Fluorosis patients having urinary fluoride above 1ppm were recruited as test group (Group-1). The
recruited fluorosis patients were sub-divided in two groups i.e., fluorosis patients with normal renal functions (G-1A) and
fluorosis paints with renal impairments (G-1B). Healthy individuals having urinary fluoride up to 1ppm were included as
control (Group-2). Urinary Exosomes were isolated and correlated with fluoride toxicity associated nephrotoxicity. A
significantly (P = 0.002) higher urinary fluoride (G-1A: 2.62 ± 0.94 ppm and G-1B: 3.87±1.6 ppm) in fluorosis patients
were observed compared to the normal control (G-2: 0.40±0.19 ppm), (P = 0.004).The serum creatinine levels was
significantly increased in G-1B (3.33±0.76 mg/dL) compared to G-1A (1.11±0.89 mg/dL), (P = 0.003) and G-2,
(0.64±0.17), (P = 0.005). A significant (P = 0.001) increase in BUN were observed in G-1B, compared to G-1A and control.
The urinary protein was also significantly higher in G-1A (P = 0.005) and G-1B (0.001) compared to G-2. The number of
exosomes per/ml of urine in G1-B was significantly higher (P = 0.05) than the G-1A and G-2. The number of urinary
exosomes in G-1A and G-1B were also significantly higher than G-2 (P = 0.05). Chronic fluoride toxicity may induce renal
impairments and increased urinary excretion of exosomes.
 
Date 2023-08-14T09:37:21Z
2023-08-14T09:37:21Z
2023-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62420
https://doi.org/10.56042/ijbb.v60i8.4300
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.60(08) [August 2023]