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Effect of cryopreservation on in vitro maturation and cleavage rate of immature prepubertal goat oocytes

Indian Agricultural Research Journals

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Title Effect of cryopreservation on in vitro maturation and cleavage rate of immature prepubertal goat oocytes
 
Creator SINHA, RAJNI
KHARCHE, S D
SINHA, N K
GOEL, A K
JINDAL, S K
SARASWAT, SONIA
 
Subject Cleavage, Cryopreservation, In vitro maturation, Prepubertal goat oocytes, Vitrification
 
Description The present study was conducted to evaluate effects of cryopreservation on the morphology, maturation rate and developmental capacity of immature prepubertal goat oocytes subjected to vitrification and slow freezing method. The proportion of immature oocytes recovered in a morphologically normal state was significantly higher for control than those for vitrification and slow freezing method. Following in-vitro maturation, the nuclear maturation rate was significantly higher for control (29.70%) than those for vitrified (9.80%) and slow freezing (18.18%) method. The percentage of normal in vitro matured oocytes following thawing in vitrification and slow freezing groups were 53.73% and 80.00%, respectively. A significant difference was observed in the cleavage rate 48 h post insemination, among vitrification, slow freezing and control were 2.0, 5.80 and 13.58%, respectively. The results indicated that the cleavage rates of oocytes in vitrification and slow freezing method did not differ significantly but differ signitificantly (P
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-09-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/33017
 
Source The Indian Journal of Animal Sciences; Vol 83, No 9 (2013)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/33017/14628
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences