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DETECTION OF ANTIGENIC VARIATION OF CANINE PARVOVIRUS STRAINS OF INDIA USING DIFFERENTIAL PCR

Indian Agricultural Research Journals

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Title DETECTION OF ANTIGENIC VARIATION OF CANINE PARVOVIRUS STRAINS OF INDIA USING DIFFERENTIAL PCR
 
Creator Manoharan, Parthiban
SARANYA, R
DIVYA, KURUNCHI C
KUMANAN, K
 
Subject Antigenic types; Canine parvovirus; Differential polymerase chain reaction; Phylogenetic analysis
 
Description Canine parvovirus 2 (CPV2) is a highly contagious and fatal disease of dogs causing acute hemorrhagic enteritis and myocarditis.  In this study, the faecal samples were initially screened using haemagglutination test followed by differential PCR assay using CPV2a and 2b antigenic type specific primers. Out of 53 samples screened, 17 samples were found to be positive by CPV2b specific primers. In PCR, the expected amplicon of 427 bp was observed in positive samples. The PCR products were sequenced and the phylogenetic analysis showed homology with CPV2b isolates. In addition to this, the presence of aminoacid aspartic acid at 426 positions clearly indicates the prevalence of CPV antigenic type 2b isolates in Tamil Nadu, India.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Date 2011-10-18
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/16073
 
Source The Indian Journal of Animal Sciences; Vol 82, No 3 (2012)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/16073/7824
 
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