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Detection of benzimidazole resistance in Haemonchus contortus populations collected from sheep of Uttarakhand using allele specific PCR assay

Indian Agricultural Research Journals

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Title Detection of benzimidazole resistance in Haemonchus contortus populations collected from sheep of Uttarakhand using allele specific PCR assay
 
Creator PANDEY, NAVEEN
VATSYA, STUTI
 
Subject Benzimidazole, Haemonchus contortus, Hill region, Resistance, Susceptible, Tarai region
 
Description Allele specific PCR (AS-PCR) was standardized to detect genotypic and allelic frequencies of Haemonchus contortusworm populations of 2 agro-climatic regions viz. Tarai and Hill regions of Uttarakhand. Genotyping of H. contortusworms revealed that overall frequency of homozygous susceptible worms were significantly higher (0.61) thanhomozygous resistant (0.17) worms. Within Tarai region, the frequency of homozygous resistant worms were significantlyhigher (0.48) in Pantnagar than homozygous susceptible (0.20) worms whereas, significantly higher frequency ofhomozygous susceptible worms were found in Haldwani (0.52) and Tanakpur (0.80). In Hills, the frequency ofhomozygous susceptible worms was significantly higher than homozygous resistant worms collected from Nainital(0.72 and 0.08 respectively) and Almora (0.84 and 0.04 respectively).Overall, the frequency of susceptible allele was significantly higher (0.72) than resistant allele (0.28). In Tarairegion, frequency of resistant allele (0.36) was significantly lower than susceptible allele (0.64). The frequency ofresistant allele was significantly higher in Pantnagar (0.64) whereas significantly higher frequency of susceptible allelewas recorded in H. contortus collected from Haldwani (0.68) and Tanakpur (0.86). In Hill region, frequency of resistantallele (0.14) was significantly lower than susceptible allele (0.86).
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-01-16
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/26390
 
Source The Indian Journal of Animal Sciences; Vol 83, No 1 (2013)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/26390/12083
 
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