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Egg yolk powder an alternative to fresh-egg yolk for buffalo semen cryopreservation

Indian Agricultural Research Journals

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Title Egg yolk powder an alternative to fresh-egg yolk for buffalo semen cryopreservation
 
Creator SINGH, MAHAK
BARIK, NIRMAL CHANDRA
GHOSH, S K
PRASAD, J K
RAJORIA, J S
SONI, Y K
KUMAR, ASHOK
CHAUDHARY, J K
SRIVASTAVA, N
 
Subject Buffalo semen, Cryopreservation, Egg yolk powder, Semen, Sperm
 
Description The present work was carried out to evaluate the effect of commercial egg yolk (EY) powder as an alternative to fresh EY on buffalo semen freezability. Ejaculates (12) from 3 bulls (4 from each bull) were collected as per standard procedure. After initial selection of ejaculates on the basis of mass activity (+3 and above) and individual progressive motility (70% and above), ejaculates were divided into 3 parts and diluted with tris dilutor, G1, containing 5% EY powder; G2, containing 10% EY powder, and G3, containing fresh EY (20%). Ejaculates were processedand cryopreserved using automated biological cell freezer followed by examination of frozen samples after 24 h. Semen quality variables (individual progressive motility, viability using propidium iodide, acrosome integrity using FITC-PSA and membrane integrity by HOST) were examined at fresh and post-thaw stage. At fresh stage semen quality variables did not vary among 3 groups.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2015-01-23
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/46163
 
Source The Indian Journal of Animal Sciences; Vol 85, No 1 (2015)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/46163/19985
 
Rights Copyright (c) 2015 The Indian Journal of Animal Sciences