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Effect of individual or group culture on in-vitro development of buffalo preantral follicles

Indian Agricultural Research Journals

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Title Effect of individual or group culture on in-vitro development of buffalo preantral follicles
 
Creator Ramesh, H S
Ravindra, J P
Gupta, P.S.P
Kumar, V Girish
 
Subject Buffalo; In vitro culture; Preantral follicles
 
Description A study was made to investigate the effect of individual (single) and group culture on in-vitro development of large preantral follicles in buffaloes. Large preantral follicles of 150 to 500 mm size isolated from slaughterhouse derived ovaries by micro-dissection method. They were placed individually or in groups in minimum essential medium supplemented with steer serum (10%), follicle-stimulating hormone (FSH-0.05 IU/ml), sodium pyruvate (0.23 Mm), glutamine (2 Mm). hypoxanthine (2 Mm), insulin-transferrin-selenium (ITS- 6.25 mg insulin, 6.25 mg transferrin, 6.25 ng sodium selenite), mercaptoethanol (10 mM/ml), gentamicin (50 mg/ml) and cultured in a CO2 incubator up to 100 days. There was no significant difference in the size of follicles between the 2 groups. However, the preantral follicles in-group culture showed a significantly higher growth, growth rate and survivability compared to those individually cultured. Thus culturing of preantral follicles in groups was beneficial for their development during in-vitro condition.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Date 2007-10-05
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/10289
 
Source The Indian Journal of Animal Sciences; Vol 77, No 10 (2007)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/10289/4693
 
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