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Typing of leptospiral serovars by DNA restriction enzyme analysis

Indian Agricultural Research Journals

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Title Typing of leptospiral serovars by DNA restriction enzyme analysis
 
Creator KUMAR, A SENTHIL
RAMADASS, P
NACHIMUTHU, K
 
Subject Rats, Leptospirosis, Leptospiral serovars, DNA restriction enzyme
 
Description DNA restriction endonucleases have been used for characterizing the various leptospiral serovars. Reference strains (13) and rat isolate RI were selected for this study. DNA samples were prepared and subjected to restriction enzyme analysis with different enzymes. Complete digestion of the samples was obtained with Bam HI, Eco RI, Hae III, Hha I and Hind III: The enzymes Hae III and Hha I produced good resolution, among the patterns produced by the enzyme Hae III facilitated clear differentiation between different serovars. The rat isolate RI restriction enzyme map was similar in pattern with the reference strain Veldrat Batavia 46 of the serovarjavanica.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2013-11-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/34578
 
Source The Indian Journal of Animal Sciences; Vol 67, No 6 (1997)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/34578/15317
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences