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Development of primary culture from gills of Tor tor (Hamilton-buchanan)

Indian Agricultural Research Journals

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Title Development of primary culture from gills of Tor tor (Hamilton-buchanan)
 
Creator KAMALENDRA, KAMALENDRA
KAPOOR, SMITA
SHARMA, J
GOSWAMI, M
RATHORE, G
LAKRA, W S
 
Subject Gill; Primary culture; Tor tor
 
Description Primary culture of gill cells was initiated from gill filament explants of game fish, Tor tor. The explants were cultured in Leibovitz L-15 medium with 5, 10 and 20% foetal bovine serum (FBS). The attachment efficiency of cells was serum-dependent, while increased FBS concentration did not stimulate further proliferation of cells. First radiation of typical gill epithelial cells was observed after 38 h, of attachment of explants. There was high proliferation for the first 7 days then a stable plateau till 10 days, followed by a decline phase from 14th day. Following removal of cells, the explants produced further cell outgrowth which was especially active at the proliferation phase (10 days). Removal of these cells caused the explants to produce a further proliferation of cells reaching confluency in 4–10 days. The use of gill explants to establish cell culture system of fish has advantages which include longevity of the culture and successive proliferations from explants, which could provide a useful tool for the investigation of toxicological and diagnostic studies.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-12-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/13356
 
Source The Indian Journal of Animal Sciences; Vol 81, No 12 (2011)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/13356/6738
 
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