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Effect of fractionated antigens of Mycobacterium bovis AN5 on blood mononuclear cells of cattle

Indian Agricultural Research Journals

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Title Effect of fractionated antigens of Mycobacterium bovis AN5 on blood mononuclear cells of cattle
 
Creator JOARDAR, S N
RAM, G C
 
Subject Culture filtrate antigen; Fractionation; Lymphoprolification; Mycobacterium bovis; Nitrite; Peripheral blood mononuclear cells
 
Description Potentiation of mononuclear immune-effector cells against mycobacteria is very much needed to prevent bovine tuberculosis. Immunopotentiating activities of various antigenic components of mycobacteria were studied previously. But knowledge regarding Mycobacterium bovis specific antigenic molecules is meagre that might be used as inducers of mononuclear immune-effector cells. With the aim of characterizing immunogenic components of M. bovis, gel filtration and anion-exchange chromatography were performed to fractionate antigens in the present study. Nitrite production by blood mononuclear cells of sensitized calves upon stimulation with fractionated antigens of M. bovis AN5 was estimated. The lymphoproliferative activity was also assessed using the similar set of antigens. One of the M. bovis specific fractionated antigens was potent inducer of both lymphoproliferation and nitrite production by mononuclear cells in vitro, than the crude antigen preparation.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-07-02
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/20218
 
Source The Indian Journal of Animal Sciences; Vol 69, No 1 (1999)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/20218/10302
 
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