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Genetic polymorphism of BoLA-DRB3.2 locus in crossbred cattle as revealed by PCR-RFLP analysis

Indian Agricultural Research Journals

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Title Genetic polymorphism of BoLA-DRB3.2 locus in crossbred cattle as revealed by PCR-RFLP analysis
 
Creator GANGULY, I
SARMA, A
DE, S
MITRA, A
 
Subject BoLA-DRB3.2, Cattle, Gcnetic polymorphism, PCR-RHLP
 
Description The present investigation was undertaken to explore DNA polymorphism in bovine class II DRB3.2 locus using polymerase chain reaction (peR) and restriction enzyme (RFLP) analysis. Genomic DNA was isolated from 21 Karan Fries (KF) and 20 Karan Swiss (KS) crossbred animals. A 304 bp fragment of the bovine lymphocyte antigen (BoLA)DRB3 gene-exon 2 was amplified by peR and the amplified product was digested independently with RsiYI, HaeIII and Rsa I enzymes. Digestion analysis revealed 3 BstYI, 6 HaeIII and 15 RsaI restriction patterns. All thesc patterns have previously been reported in cattle. In addition analysis of combined patterns of the 3 enzymcs resulted in 4 probable new alleles, viz. kba, tbb und tbe in KF and dba in KS.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2014-03-02
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/38343
 
Source The Indian Journal of Animal Sciences; Vol 74, No 2 (2004)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/38343/17237
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences