Comparison of efficiency of parasitological methods with Ag-ELISA in Trypanosoma evansi infected crossbred calves
Indian Agricultural Research Journals
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Title |
Comparison of efficiency of parasitological methods with Ag-ELISA in Trypanosoma evansi infected crossbred calves
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Creator |
SINGH, A
CHAUDHRI, S S |
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Subject |
Ag-ELISA, Calves, Parasitological methods, Trypanosoma evansi, Trypanosomosis
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Description |
The efficiency of parasitological methods including wet blood film (WBF), microhaematocrit centrifugation technique (MHCT) and mouse sub-inoculation test, (MSI) was compared with the polyclonal antibody based indirect antigen detection enzyme immunoassay (Ag-ELlSA) in 8 to 12 months old crossbred male calves experimentally infected with Trypanosoma evansi. The parasitaemia was found in 3 crossbred calves on day 3 post infection (PI) and in remaining calf on day 7 PI with MHCT and WBF. The antigenaemia was detectable with Ag- ELISA for the first time in all infected calves on day 7 PI. Thereafter, antigenaemia was detectable throughout the post infection (PI) periods while parasitaemia continued to be detected at irregular periods. Ag-ELISA was more sensitive than parasitological tests in obscrving the true epidemiological status of trypanosomos is in cattle.
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Publisher |
Directorate of Knowledge Management in Agriculture
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Contributor |
—
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Date |
2014-02-04
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Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion — |
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Format |
application/pdf
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Identifier |
http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/37403
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Source |
The Indian Journal of Animal Sciences; Vol 72, No 2 (2002)
0367-8318 |
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Language |
eng
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Relation |
http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/37403/16759
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Rights |
Copyright (c) 2014 The Indian Journal of Animal Sciences
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