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Is quantitative PCR (qPCR) an effective method to count larvae

CSIRO RDS Repository

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Title Is quantitative PCR (qPCR) an effective method to count larvae
 
Creator Pascal Craw
 
Subject Conservation and biodiversity
Marine and estuarine ecology (incl. marine ichthyology)
 
Description Currently the only way to determine the number of coral larvae in a plankton sample is to manually count the larvae under microscope.
The dataset contains information from a trial to test the suitability of quantitative PCR to measure larval number.
Prior to the start of each spawning season a single species of coral was placed in a rearing tank at the Heron Island Research Station. Other tanks were prepared in parallel with different species (when available). The number of species per spawning season ranged from 2 to 4 species.
Each day post spawning event a set number of larvae were removed from the tank (e.g. 1 larvae, 10 larvae, 100 larvae) and placed into 2mL tubes. There were 5 replicates per count. Samples of tank water were also taken each time larvae were removed from the tank. All samples were preserved at -20⁰C for DNA extractions.
This process was repeated over 4 spawning seasons (2019, 2020, 2021 and 2022).
DNA was extracted from the larval samples and from the tank water samples. The DNA was then run through a quantitative PCR (qPCR) assay designed specifically for coral larvae. The output is expressed as the number of copies of the cytochrome oxidase I gene in an individual larvae at a particular age.

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Metadata is fully public. Data files will be made fully public once the research has been published.
For special requests, please directly contact the Data Custodian and CC the Project Leader.
 
Publisher CSIRO
 
Contributor Bronwyn Holmes
Cynthia Riginos
 
Date 2023-08-29
 
Type —
 
Format —
 
Identifier csiro:60109
 
Language —
 
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