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Association of Beeta-casein gene polymorphism with milk production traits in buff810 (Bubalus bubalis)*

Indian Agricultural Research Journals

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Title Association of Beeta-casein gene polymorphism with milk production traits in buff810 (Bubalus bubalis)*
 
Creator Singh, Sanjeev
Kumar, Pushpendra
Bhattacharya, T K
Bhushan, Bharat
Sharma, Arjava
 
Subject Beeta-casein; Buffalo; Gene polymorphism; Milk production traits
 
Description Milk proteins consist of casein and whey proteins. The investigation was carried out to detect the polymorphism in Beeta-casein gene at the DNA level and study its association with milk production and constituent traits. Lactating animals (200) belonging to Murrah, Bhadawari, Surti and Mehsana breeds were included. Genomic DNA was isolated from blood samples and used to amplify 465 bp fragment of Beeta-casein gene (partial exon 7) by PCR. PCR- SSCP technique was used to study the variability in this fragment. The fragment was polymorphic. In 465 bp fragment, 4 genotypes, viz. AA, AB, AC and BB were identified in different breeds. The genotypic frequencies of AA, AB, AC and BB genotypes were 0.36,0.56,0.04 and 0.04, respectively, in Mehsana breed. In Murrah, the frequency of AA, AB and AC genotypes was 0.10,0.80 and 0.10, respectively. Bhadawari breed had AA and AC genotypes with genotypic frequencies as 0.96 and 0.04, respectively, while in Surti, AA and AB genotypes had 0.68 and 0.32 as their genotypic frequencies, respectively. The least square analysis for the association study of 465 bp fragment showed significant effect of AB genotype on total SNF yield only in Surti buffaloes. The information generated by this study can be utilized for identification of animals with superior alleles.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Date 2007-08-05
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/10370
 
Source The Indian Journal of Animal Sciences; Vol 77, No 8 (2007)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/10370/4771
 
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