Record Details

Molecular cloning of IFN-alpha in goat (Capra hircus) and black buck (Antelope cervicapra) and evaluation of its expression in goat PBM cells

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Molecular cloning of IFN-alpha in goat (Capra hircus) and black buck (Antelope cervicapra) and evaluation of its expression in goat PBM cells
 
Creator D, RAMESH
SAINI, MOHINI
SWARUP, DEVENDRA
SINGH, VIVEK KUMAR
UPRETI, SUCHITRA
DAS, ASIT
GUPTA, PRAVEEN K
 
Subject Antelope cervicapra; Capra hircus; Interferon alpha; PBM cells; Real time PCR; TLR9
 
Description Type I interferons (IFN-α/ß), play a pivotal role linking innate and adaptive immune responses, of which interferon (IFN- α) alpha (leukocytic interferon) that was initially known for antiviral effect, but in recent days recognized as major key elements in priming innate immune response. Bacterial DNA, rich in CpG motifs, triggers the toll-like receptor-9 mediated signaling through IFN- α. The present study reports cloning and characterization of full length ORF of IFN- α in goat and black buck and its expression profile in goat peripheral blood mononuclear cell culture in presence of Pasteurella multocida DNA. The open reading frame of IFN-α in these species was 570 bp as in other ruminant species. The expression of IFN- α in presence of Pasteurella multocida DNA (rich in CpG motifs) was higher as compared to unstimulated cells. It is plausible that pasteurella DNA induced TLR9 expression, which contributed to rise in the IFN- α transcript in cultured PBM cells of goat.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-01-02
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/13834
 
Source The Indian Journal of Animal Sciences; Vol 82, No 1 (2012)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/13834/6868
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences