Record Details

Development of a non-radioactive probe generated by RAPD-PCR for the detection of Theileria annulata

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Development of a non-radioactive probe generated by RAPD-PCR for the detection of Theileria annulata
 
Creator SARAVANAN, B C
BANSAL, G C
MANIGANDAN, L
SANKAR, M
RAVINDRAN, R
RAO, J R
 
Subject DNA probe; RAPD-PCR; Theileria annulata
 
Description A highly reproducible, dominant and monomorphic fragment of 963 base pair (bp) was amplified from the genome of Theileria annulata by random amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR) using a arbitrarily selected primer AP₁₇ (5’-GGTGACGCAG-3’). This fragment was reamplified using the same random primer (AP₁₇), gel purified and labeled with digoxigenin (DIG). Dot-blot hybridization of total genomic DNA with the probe detected Parbhani and Izatnagar strains of T. annulata with a threshold detection level of 10 pg of parasite template DNA. No cross-hybridization was observed with Babesia bigemina, Trypanosoma evansi and the bovine host DNA. The sensitivity of the DIG labeled probe developed in this study is at least 6 to 65 times higher than the other DIG labeled probes developed in India and therefore considered highly suitable for diagnosis of carrier animals especially when they are imported or exported from the country.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2011-11-14
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/12649
 
Source The Indian Journal of Animal Sciences; Vol 81, No 11 (2011)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/12649/6322
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences