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Development of primary culture from heart tissue of Chitala chitala (Hamilton-Buchanan)

Indian Agricultural Research Journals

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Title Development of primary culture from heart tissue of Chitala chitala (Hamilton-Buchanan)
 
Creator KAPOOR, SMITA
KAMALENDRA, KAMALENDRA
P, BHATT J
S, LAKRA W
G, RATHORE
M, GOSWAMI
SWAMINATHAN, T RAJA
 
Subject Chital; Heart; Primary culture
 
Description A method for developing cell cultures from Chitala chitala heart tissue is described. The tissues were minced and seeded in culture flasks and grown in Leibovitz L-15 medium supplemented with 20% foetal bovine serum. The radiation started after 4th day in heart. The medium was changed after every five days. The radiations were able to withstand a wide range of temperatures from 25 °C to 28°C with an optimum temperature of 28°C. A partial monolayer was observed after 12 days in heart tissue. A primary culture was successfully obtained from heart tissue of C. chitala for the first time in India.
 
Publisher Directorate of Knowledge Management in Agriculture
 
Contributor —
 
Date 2012-01-02
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
—
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/13869
 
Source The Indian Journal of Animal Sciences; Vol 82, No 1 (2012)
0367-8318
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/13869/6890
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences